12th International Conference on Harmful Algae

More documents

Recommendations

Info

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006R. Wayne Litaker, Patricia TesterNational Ocean Service, NOAA,BEAUFORT, NC, United States of AmericaDinoflagellate taxonomy is based onmorphological criteria that areinherently more difficult to obtainthan are corresponding DNAsequence data. This results in therapid accumulation of sequenceinformation indicating many morespecies exist than can beadequately described given theextant morphological data.Currently, no method exists forsystematically categorizing thesesequences into potential speciesgroups for use in ecological studiesor for taxonomic characterization.We examined whether geneticdistances among ITS1/5.8S/ITS2rDNA sequences could be used forthis purpose. The analysis involvedidentifying sequences from 81dinoflagellate species to determineif simple uncorrected geneticdistances (p) above a certain levelconsistently correlate with knownspecies boundaries. For a diverseassemblage of dinoflagellatespecies, the within species geneticdistances between ITS1/5.8S/ITS2copies (p=0.000-0.021 substitutionsper site) were consistently less thanthose observed between species(p=0.042-0.580). Our resultsindicate that a between speciesuncorrected genetic difference>0.04 could be used to delineatemost dinoflagellate species,including cryptic forms. Recentlyevolved species, however, mayhave ITS p values
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006donghaiense, and that N-phenyl-2-naphthylamine and linoleic acidmight play an important role in theinhibition.PO.05-44Production of monoclonalantibody and development of anenzyme-linked immunosorbentassay for the determination ofokadaic acid in shellfishSession: PO.05 - MonitoringRen-Yan Liu 1 , Bing-Jun Chen 2 , Dao-Yan Xu 3 , Yu-Bo Liang 4 , Bing Liang 31 Dalian Maritime University, DALIAN, China2 Dalian Fisheries University, DALIAN,China3 Natl. Marine Environ. Monitoring Centre,DALIAN, China4 SOA Key Lab of Coastal Env. & EcosysRes, DALIAN, ChinaAn indirect competitive enzymelinkedimmunosorbent assay forquantitation of okadaic acid(OA), amarine biotoxin associated redtides, was developed by preparationof a monoclonal antibody againstOA using the cell-fusing method.OA-KLH was used as immunogen,and the spleen cells of immunizedmice were fused with Sp2/O cells.Clones secreting specificmonoclonal antibodies werescreened by indirect ELISA usingOA-OVA as coating ligand. Aftercloning, three hybridoma cell clonesstably producing anti-OAmonoclonal antibody were obtained.Detection of OA concentrations byindirect competitive ELISA wasestablished. The detection limit ofOA was 0.781ng/ml. The level ofOA in shellfish from China wasdetermined using the idc-ELISAmethod. A good correlation(R=0.7963,P‹ 0.001) was observedbetween idc-ELISA and HPLC-MS/MS.PO.10-28Response to small-scaleturbulence by naturalmicrophytoplanktonassemblages along a naturalAlexandrium minutum bloomeventSession: PO.10 - Ecophysiology &autecologyG Llaveria, E Garcés, E Berdalet, NSampedro, S Anglès, Ò GuadayolInstitut de Ciències del Mar, BARCELONA,SpainSmall-scale turbulence is anenvironmental factor that maydirectly affect different physiologicalprocesses of phytoplankton. Mostavailable data have been generatedin the laboratory using monospecificphytoplankton cultures. In thepresent work we used field samplesof a bloom of dinoflagellatesdominated by Alexandriumminutum, Scrippsiella spp. andProrocentrum micans, in differentphases of the succession, and wetested their sensitivity toexperimentally generated smallscaleturbulence.Three experiments, each 15 daysapart, were performed on themicroplankton fraction (15-60 µm) ofthe natural water. The sampleswere distributed in 4-liter sphericalflasks and maintained still orexposed to turbulence generated byan orbital shaker (turbulentdissipation rate epsilon~15 cm 2 s -3 )for ca. 10 days. The response wasevaluated in terms of biomass yield,net growth rate and cyst abundanceof the dominant species.Diatoms, A. minutum andScrippsiella spp. collapsed understill conditions while P. micansgrowth was not favoured byturbulence. Temporary cystformation of A. minutum was224
Page 1 and 2:
INTERNATIONAL SOCIETYFOR THE STUDY
Page 3 and 4:
Dear participantWelcome to Denmark
Page 5 and 6:
INTERNATIONAL SOCIETY FOR THE STUDY
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174: INTERNATIONAL SOCIETY FOR THE STUDY
Page 223: INTERNATIONAL SOCIETY FOR THE STUDY
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
Page 333 and 334:
Page 335 and 336:
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
show all

12th International Conference on Harmful Algae

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?