12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006induced ecological restoration),which made it possible to solve allthese problems. In the study of theMLS technique, it was found thatsome natural netting and bridgingmaterials, such as chitosan, turnedmany solids, such as localsoils/sediments, into highly effectiveflocculants in removingcyanobacterial blooms fromn freshwaters (1-3). When MLS was furthermodified with macrophyte seeds(MLSIER), the short-term effect ofalgal removal triggered a long-termmacrophyte growth (the latter wouldotherwise die in HAB waters), whichpermanently fixed the cells andnutrients into the sediments.1. Pan G, Zhang MM et al.,Environmental Pollution, 2006, 141(2): 195.2. Zou H, Pan G et al.,Environmental Pollution, 2006, 141(2): 201.3. Pan G, Zou H et al.,Environmental Pollution, 2006, 141(2): 206.PO.10-18Trying to cultivate Dinophysisacuminata, a dinoflagellatecausing diarrhetic shellfishpoisoningSession: PO.10 - Ecophysiology &autecologyMG Park 1 , S Kim 2 , HS Kim 3 , YG Kang 2 ,W Yih 21 Chonnam National University, GWANGJU,South Korea2 Kunsan National University, KUNSAN,South Korea3 Gunsan RMAFO, MOMAF, KUNSAN,South KoreaThe dinoflagellate genus Dinophysisincludes several toxic species whichcause diarrhetic shellfish poisoning,and no species of the genus has yetbeen established in culture. Whilethe plastids in Dinophysis areknown to be of cryptophyte orhaptophyte origin, whether they arekleptoplasts and how they areacquired by Dinophysis still remainunresolved. To address theseissues, we tried to establish D.acuminata in laboratory culture. Theresults will be discussed in contextof biology and plastid evolution ofDinophysis species.PO.12-08Morphological characteristicsand life cycle of the diatomThalassiosira cf stellarisSession: PO.12 - Taxonomy and phylogenyJG Park, J RenKunsan Univ., JEOLLABUK-DO, SouthKoreaStrains of Thalassiosira stellaris andThalassiosira cf stellaris wereisolated off the coast of Wando,Korea. T. cf stellaris resembles T.stellaris in many morphologicalcharacteristics such as cell length,fascicular distribution of areolae,and the number and interval ofstrutted processes on the marginand middle of the valve face. T. cfstellaris differs in lacking dot-shapedsmall processes on the valve face,and the strutted processes aresurrounded by four additional smallareolae. T. cf stellaris displayedtypical oogamous reproduction. In aphase of vegetative multiplication,several cells were connected bymucous threads to build up shortchains in which each cell standsapart at regular intervals, but duringand/or just before sexualreproduction, the cells were closelyconnected to neighbouring cellsproducing chains withoutmucilaginous threads. Cell counts ofsingle cells decreased with growthof the filamentous chains. These257
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006results imply that the filamentouschains might play the role of oocyteand the single cells may bespermatocytes. In spite of identicalculture conditions of T. cf stellarisand T. stellaris, sexual reproductionwas observed only in T. cf stellaris.PO.10-41Examination of the cell cycle,growth rate, and meiosis ofKarlodinium spp. by flowcytometrySession: PO.10 - Ecophysiology &AutecologyMW Parrow 1 , JM Burkholder 2 , EGarcés 31 University of North Carolina Charlotte,CHARLOTTE, United States of America2 Center for Applied Aquatic Ecology,RALEIGH, United States of America3 IRTA, Inst. de Recerca i Tecnol. Agroal.,SANT CARLES DE LA RÀPITA, SpainThe DNA content, DNA synthesiscycles, and growth rates of differentstrains of Karlodinium veneficumand K. armiger were studied inculture by flow cytometry. A 3-folddifference in DNA content wasfound between K. veneficum and K.armiger, indicating a significantdifference in genome size. K.veneficum strains had a dielperiodicity of DNA synthesis (S) andcell division (G2M) that was phasedwith the photocycle. Cells with 1CDNA (G1) began DNA synthesis (S)during the latter half of the lightperiod, then entered G2M anddivided during the dark period.Variation among K. veneficumstrains was found in the duration ofthe S phase (3.2-8.4 h) and G2Mphase (2.0-12.7 h). Potential growthrates derived from cell cycle dataranged from 0.55 to 1.03 divisionsday -1 , and were higher than netgrowth rates calculated from cellnumbers. In most strains, a distinctsubpopulation of cells with a singlenucleus containing 4C DNA alsooccurred during the dark period.Based on observations of thesexual life cycle of K. veneficum, itis proposed that these 4C DNA cellswere zygotes undergoing meiosis.This potentially co-occurring meioticcycle could impact determinationsof the mitotic cell cycle ofKarlodinium.PO.08-20Impacts of the toxicdinoflagellate Alexandriummonilatum on three ecologicallyimportant shellfish speciesSession: PO.08 - ToxicologySE Pate 1 , JM Burkholder 1 , SEShumway 21 North Carolina State University, RALEIGH,United States of America2 University of Connecticut, GROTON,United States of AmericaAlexandrium monilatum is a toxicdinoflagellate that forms bloomsmostly in the Gulf of Mexico. ToxicA. monilatum has been linked to fishand invertebrate kills, and producesendotoxins with hemolytic andneurotoxic properties. Weexperimentally assessed responsesof ecologically important shellfish totoxic A. monilatum (AMO3 isolate).Grazing studies were conductedwith eastern oysters (Crassostreavirginica), northern quahogs(Mercenaria mercenaria) and greenmussels (Perna viridis), all of whichinhabit areas where A. monilatumblooms occur. Clearance rates ofeach shellfish species weredepressed when exposed to toxic A.monilatum alone or with nontoxicInstant Algae® Pavlova, incomparison to control animals thatwere fed benign algal prey.Exposure to A. monilatum alsosignificantly decreased shellfish258
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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