12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.01-19Distribution and diversity oftoxigenic Microcystis blooms: atemperate-tropical comparisonSession: PO.01 - GeneticsWGA Vyverman 1 , I van Gremberghe 1 ,T Asmelash 2 , T Dejenie 2 , J vanWichelen 1 , K van der Gucht 1 , L deMeester 3 , A Wilmotte 41 Ghent University, GENT, Belgium2 Mekelle University, MEKELLE, Ethiopia3 Catholic University Leuven, LEUVEN,Belgium4 Université de Liège, LIÈGE, BelgiumCyanobacterial blooms wereinvestigated in shallow lakes andponds in Belgium and microdams inEthiopia during respectively threeand two consecutive years. DGGEprofilingof 16S rDNA revealed thatthe most dominant bloom-forminggenus was Microcystis in bothcountries. DGGE-profiling ofMicrocystis-specific16S-23S rDNAITS revealed a significant diversitywithin and among sites. PCR wasused to detect the presence of mcygeneswithin blooms and revealedthat most blooms containedtoxigenic strains. Phylogeneticanalysis of ITS-sequences wasused to relate toxigenic capacity tothe genetic composition of blooms.Multivariate analyses were used torelate bloom composition to climaticcondition, nutrient status andbiological characteristics.PO.15-02The potentially harmful algae andHABs in the East China Sea byregular red tide monitoringprogrammeSession: PO.15 - MonitoringJinhui Wang, R Xu, XS Cheng, XQHuangEast China Sea Environmental Monitoring,SHANGHAI, ChinaAbout 435 red tides are recorded inthe East China Sea since the1930s, which amounts to 54% oftotal red tides in China. Amongthem, 43 toxic HABs were recorded;the causing species includeAlexandrium tamarense, Kareniamikimotoi, Heterocapsacircularisquama, Cochlodinium sp.and Karenia brevis. The HABs ofKarenia mikimotoi in 2005 causeddirect loss of about RMB 20 millionby killing cultured fish (red drum,genuine porgy) and abalone. Thereare about 24 recorded blooms ofKarenia sp. in the East China Seasince 1980s, most of them havedetrimental influence, and thebloom of Karenia sp. has atendency to increase in frequencyand to be spreading from south tonorth. According to regularphytoplankton monitoring since2002, there are 19 potentially toxicalgae: PSP-causing algae(Alexandrium catenella,Alexandrium tamarense); DSPcausingalgae such as Dinophysiscaudata, Dinophysis fortii,Dinophysis acuminate; NSPcausingalgae, Karenia brevis, ASPcausing algae such as Pseudonitzschiamultiseries, Pseudonitzschiamultistriata, Pseudonitzschiadelicatissima, and other 8toxic algae such as Heterocapsacircularisquama, Cochlodinium sp.,Fibrocapsa japonica etc. Amonitoring scheme of toxic algaeand biotoxin is put forward.PO.02-04A proteomic approach to harmfulalgal bloom researchSession: PO.02 - GenomicsDa-Zhi Wang 1 , Hua-Sheng Hong 1 , LeoLai Chan 2 , John Hodgkiss 21 Xiamen Univeristy, XIAMEN, China301
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 20062 Hong Kong University, HONG KONG,ChinaProteomics, defined as the study ofproteins expressed by the wholegenome at the setting time andconditions, has been applied in thestudying of harmful algal bloom(HAB) research and has shown highpotential for identification ofspecies-specific proteins for speciesidentification; toxin biomarkers forstrain differentiation; physiologicalindicator proteins to rapidly assessthe nutritional or metabolic status ofcausative agents of HAB forprediction, detection, monitoring andverification of bloom events; and cellsurface associated proteins to studythe interaction betweenphytoplankton and theirenvironment as well as to separatespecies from mixed phytoplanktonpopulations. The paper will presenta review of the various techniqueswe applied; namely 2-DE, 2-DDIGE, MALDI TOF MS/MS, N-terminal sequencing andimmunoblotting; and the results wehave obtained in HAB studies.PO.16-02Distribution of dinoflagellateresting cysts in surfacesediments from ChangjiangEstuary in the spring of 2004Session: PO.16 – Life cyclesZH Wang, YZ Qi, YF YangJinan University, GUANGZHOU, ChinaSurface sediments were sampled inApril and May of 2004 fromsouthern Changjiang Estuary,where a large- scale Prorocentrumdonghaiense red tide accompaniedby Alexandrium sp. occurred inearly May of 2004, in order to studycyst composition and distributionbefore and during the red tide.Results showed only fewdifferences in cyst compositionbetween the two surveys. Theaverage cyst concentrations in Apriland May were 374 and 482 cystsper gram D.W. sediment,respectively. Cysts of Alexandriumoccurred commonly but in lownumbers, and the maximumconcentration during the bloom was219 cysts per gram D.W. sediment.High sand content in sediments,high sedimentary rate, huge dilutedwater and complicated watercurrents, are thought to result in thelow cyst assemblages inChangjiang Estuary. Thedestination of the Alexandrium cystsformed after the bloom is discussedas.PO.06-10The interspecific competition oftwo HAB species: Prorocentrumdonghaiense and AlexandriumtamarenseSession: PO.06 - Population dynamicsZL Wang, RX Li, MY ZhuFirst Institute of Oceanography, SOA,QINGDAO, ChinaWe studied the population dynamicsand interspecific competition ofProrocentrum donghaiense andAlexandrium tamarense, twocommon HAB species in East ChinaSea, by using semi-continuousdilution experiments. Prorocentrumdonghaiense was the winner at lowphosphate concentrations, whichcompletely excluded Alexandriumtamarense. However, at highphosphate conditions, thecompetition between the twospecies was different, and eithercaused exclusion of one species orcoexistence. The results also showthat the competition results dependon the cell densities which the two302
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12th International Conference on Harmful Algae

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