12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006veneficum 71-83%). The relativelevels of the PUFA OPA and DHA,coupled with the potential inhibitoryaction of Δ8(14) sterols, and thepotential by many of these speciesto produce reactive oxygen species,may provide insight into theichthyotoxicity of these bloomformingdinoflagellates.PO.15-32Utilization of volunteers tomonitor harmful algal blooms inthe southeastern coast of theUnited StatesSession: PO.15 - MonitoringSteve L MortonNOAA/NOS, CHARLESTON, SC, UnitedStates of AmericaThe Southeast PhytoplanktonMonitoring Network (SEPMN) wasestablished by the National Oceanic& Atmospheric Administration(NOAA) as an outreach program tounite volunteers and scientists inmonitoring harmful algal blooms.Currently, there are 60 volunteergroups that monitor 72 sites alongthe southeast Atlantic coast fromnorthern Outer Banks, NC toJacksonville, FL.The Program is based on avolunteer network of schools,community groups, andparks/recreational facilities.Volunteers are instructed on algalidentification and sample on aweekly/biweekly basis, reportingtheir data to researchers at theMarine Biotoxins Program. Resultsfrom volunteer groups enableresearchers to identify problemareas to isolate for further study. Avolunteer based monitoring networkenables researchers to maintainand monitor an extended surveyarea throughout the year.Since 2001, SEPMN has providedopportunities for teachers, students,and community members toparticipate in real scientificresearch, helping to expandknowledge about phytoplankton andthe roles these organisms play inour daily lives. For more informationabout SEPMN, visit the website:http://www.chbr.noaa.gov/PMN/.PO.12-02Species of the genus PseudonitzschiaPeragallo(Bacillariophyceae) in Greekcoastal watersSession: PO.12 - Taxonomy and phylogenyK Moschandreou, G NikolaidisAristotle University of Thessaloniki,THESSALONIKI, GreeceRecords of species belonging to thepotentially toxic (Amnesic ShellfishPoisoning) diatom genus Pseudonitzschiain Greek coastal watersare limited. This study presentsdetailed information about theoccurrence and morphologicalvariability of Pseudo-nitzschiaspecies in Greek coastal waters. Ataxonomic survey was carried out atthe main harvesting and shellfishgrowing areas 2004-2006. Speciesidentification was based on light(LM) and electron (SEM and TEM)microscopy of field samples(preserved and live material) andunialgal cultures. Eight species/taxawere identified; P. fraudulenta, P.multiseries, P. multistriata, P.pungens and four species within theP.pseudodelicatissima/cuspidatacomplex: P. caciantha, P. calliantha,P. pseudodelicatissima, and amorphospecies that closelyresembles what is described as P.caciantha (Pseudo-nitzschia cf.caciantha). Morphometric data aregiven and the observed245
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006morphological variability of thespecies within P. pseudodelicatissima/cuspidatacomplex isdiscussed. The spatial distribution ofthe various species is also outlined.PO.09-02Enhancement of gymnodimineproduction in automated cultureof Karenia selliformisSession: PO.09 - Toxin synthesis andchemical structure of toxinsDO Mountfort, V. Beuzenberg, LMacKenzie, P HollandCawthron, NELSON, New ZealandGymnodimine has recently attractedinterest because of itspharmacologically interestingproperties mainly residing in thespiro-cyclic imine ring, which isthought to activate L-type calciumchannels of brain receptors. Inorder to investigate itspharmacology, a requirement forthe production of gymnodimine isthe guarantee of a reliable andenhanced supply of the compound.We have constructed aphotoreactor (15 L capacity)allowing improved light access (190μmol/m 2 /s) operated in a 12hlight:12h dark cycle, with gentlestirring and aeration of culture. Theunit also has an inlet for carbondioxide injection, and capability ofpH and turbidometricmeasurements. We have operatedthe reactor on a semi-automaticbasis with pH poised at 8.5. Growthkinetics and gymnodimineproduction by K. selliformis (growthyield maximum, 46510 cells/ml;doubling time, 8.6 days;gymnodimine, 0.5 μg/ml) weresubstantially improved compared tostandard (12 L) batch culture(growth yield max. 41,510 cells/ml;doubling time, 17.3 days;gymnodimine, 0.16 μg/ml) with nopH amendment. As a consequencea fully automated culture systemhas been developed. We describeparameters for growth andgymnodimine production byK.selliformis with respect to fixedculture pH in this system.PO.10-27Comparison of growth rate andefficiency of the Texas brown tidealga Aureoumbra lagunensiswhen grown on DON and DINSession: PO.10 - Ecophysiology &autecologyHI Muhlstein 1 , TA Villareal 21 The University of Texas at Austin,DAUPHIN ISLAND, ALA, United States ofAmerica2 The University of Texas, PORTARANSAS, TX, United States of AmericaThe nuisance Texas brown tide(TBT) alga, Aureoumbralagunensis, reached bloomdensities once again beginning inthe spring of 2005. Although theoriginal Texas brown tide bloompersisted 1990-1997, there is onlylimited information on the growth ornutritional characteristics of thecausative organism. Weinvestigated the effect that organicnitrogen availability and reducedphotosynthetically availableradiation (PAR) have on the growthof TBT. Such parameters seemcritical for blooms of themorphologically and geneticallysimilar east coast brown tidespecies Aureococcusanophagefferens. We tested theability of TBT to utilize DIN (NO 2 - orNH 4 + ) and DON (urea or glutamicacid) as the sole source of nitrogenunder 8 light intensities ranging fromlimiting to saturating irradiancesusing a light gradient table. Theexperiments demonstrated highest246
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12th International Conference on Harmful Algae

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