12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006circularisquama is a temporarystage rather than a resting stage.Heterocapsa circularisquama grewwell at ca.17.5 °C, poorly at 15 °Cand 12.5 °C, and it died at 10 °C.Two weeks after inoculation, theratio of pellicle cyst/total cells at12.5 °C was 57.5±1.4 %,significantly higher than at the othertemperatures. After 60 days and 90days of incubation at 10 °C undercontinuous darkness, viabilitypercentage of the pellicle cyst was0.9% and 0.8 %, respectively. Thus,the pellicle cyst designated astemporally cyst in the otherdinoflagellates is not always in atemporary stage in H.circularisquama, part of a H.circularisquama population mightoverwinter as pellicle cysts formedat low temperature.PO.13-25Long-term variation ofphytoplankton in Harimanada,Seto Inland Sea, JapanSession: PO.13 - Regional eventsSadaaki YoshimatsuAkashiwo Res. Inst. of Kagawa Pref.,TAKAMATSU, JapanChattonella antiqua wasresponsible, in the 1970-1980s, forlarge fishery damages inHarimanada, Seto Inland Sea,Japan. PSP toxicity is also aproblem in this region. Thisresearch was carried out to clarifythe appearance of phytoplankton,including harmful and toxic species.Long-term variations inphytoplankton a point inHarimanada were investigated fromApr. 1983 to Dec. 2005, three to fivetimes a month during summer, andone to three times a month at otherseasons. A litre of seawater wascollected from 8 layers at 5-mintervals, filtered and concentratedto 50 mL. The number ofphytoplankton species in theunfixed samples was counted.Among the species observed in thisstudy, harmful and toxic speciesfound were: Chattonella antiqua, C.marina, C. ovara, C. verruculosa,Heterosigma akashiwo, Kareniamikimotoi, Cochlodiniumpolykrikoides, Heterocapsacircularisquama, Alexandriumcatenella, A. tamarense,A.tamiyavanichii, Gymnodiniumcatenatum and Dinophysis fortii.PO.05-20Preparation of toxin standardsfor use in monitoring diarrheticshellfish toxins by LC-MSSession: PO.05 - Toxin analysisA YoshinoTropical Technology Center Ltd., URUMA-CITY, JapanIn determining marine toxins inseafood, LC-MS is the mostpowerful tool with its high sensitivity,specificity, and accuracy. Topromote wide use of the method,however, an adequate supply ofstandard toxins is imperative.Though highly or moderatelycontaminated shellfish have beenunavailable for years in Japan, wesucceeded in preparing DSP-toxinstandards by taking differentapproaches. First, we obtainedokadaic acid (OA) anddinophysistoxin-1 (DTX1) byculturing Prorocentrum lima.Second, OA and DTX1 wereextracted from the black sponge,Halichondria okadai. Third, in orderto secure DTX1, which was of lowoccurrence in the preceding twosources, we improved purificationprocedures to enable purification ofDTX1 from scallop extracts of very309
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006low DTX3 contents. Fourth, weimproved reaction conditions tosynthesize from OA and DTX1corresponding 7-O-palmitoyl esters.The structure and purity of thestandard toxins thus prepared wereconfirmed by HPLC-DAD, 1D- and2D-NMR, and ESI-MS. The toxinquantity in a parent stock wasdetermined by weight.impact on the lake, such as deadfish, shrimp or water plants.This represents a successfulexample of mitigation of lakeblooms. It improved the waterquality for the benefit of theresidents and ensured that theaquatic sport activities of the '10thnational games' could proceednormally as scheduled.PO.14-02A successful control of HABs bymodified clay: mitigation ofCyanophyta blooms in XuanwuLake in NanjingSession: PO.14 - MitigationZM Yu 1 , XX Song 1 , XH Cao 1 , ZHZhang 21 Institute of Oceanology, CAS, QINGDAO,China2 Centre of Environment Monitoring,NANJING, ChinaA bloom of Microcystis aeruginosaoccurred in Xuanwu Lake in June,2005, which had serious impact onlandscape, water quality and theliving conditions of residents. It alsoposed as a threat to aquatic sportactivities of '10th national games',scheduled to take place in the lake.Based on the theory of clay surfacemodification, clays with highcoagulation efficiency were madeand disseminated in different ratios,frequency and concentration,matching the distributioncharacteristics of the blooms indifferent lake areas. The bloomswere controlled effectively.Monitoring showed that the averageremoval rate of Microcystisaeruginosa was more than 96% andthe coagulated cells weredecomposed gradually. The qualityof lake water had significantlyimproved. The community structureof phytoplankton had changed.There was no visible negativePO.05-33Analysis of toxins responsible forpoisoning incidents caused byconsumption of the snailNassarius spp.Session: PO.05 - Toxin analysisRC Yu 1 , AF Li 1 , J Li 1 , YF Wang 1 , MJZhou 1 , T Yan 1 , M Quilliam 2 , B Luckas 31 Institute of Oceanology, CAS, QINGDAO,China2 Institute for Marine Bioscience, NRC,HALIFAX, Canada3 Jena University, JENA, GermanyDuring 2002 and 2003, poisoningincidents caused by theconsumption of snail Nassarius spp.were reported in Fujian Provinceand Jiangsu Province, China. Afterobservation of the symptoms in thevictims it was suspected that PSPtoxins were involved, and thetoxicity screening of the snailsamples with mouse bioassaymethod for monitoring of PSP toxinsgave numbers exceeding10,000MU/100g tissue (wet weight).However, no PSP toxins weredetected after analysis of snailsamples using high performanceliquid chromatography (HPLC) withpost-column derivatization. Thetoxins in Nassarius spp. were finallyidentified as tetrodotoxin (TTX),after re-analysis of the samplesusing HPLC coupled with a massdetector. The derivatives of TTX intoxic snail samples were alsoanalyzed, using HPLC coupled with310
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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INTERNATIONAL SOCIETY FOR THE STUDY
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12th International Conference on Harmful Algae

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