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12th International Conference on Harmful Algae

12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <str<strong>on</strong>g>Internati<strong>on</strong>al</str<strong>on</strong>g> <str<strong>on</strong>g>C<strong>on</strong>ference</str<strong>on</strong>g> <strong>on</strong> <strong>Harmful</strong> <strong>Algae</strong>, Copenhagen, Denmark, 4-8 September 2006occurrences of algal groups;including cyanobacteria. In thepresent study the compliancebetween this strategy and othermethods comm<strong>on</strong>ly applied todescribe and quantify phytoplankt<strong>on</strong>compositi<strong>on</strong> were compared;including lab fluorometry,spectrophotometry and HPLCpigment analyses andmicroscopically examinati<strong>on</strong>. Inadditi<strong>on</strong>, the occurrence ofmicrocystins was assessed. Ingeneral, acceptable coherence wasobserved between the differentstrategies when accessed based <strong>on</strong>the occurrence and dominance ofcyanobacteria. Regarding the otheralgal groups obvious discrepanciesoccurred; i.e. the in situ fluorometermethod was not able to describe thephytoplankt<strong>on</strong> community properly.O.19-05Phytoplankt<strong>on</strong> communitycompositi<strong>on</strong> observed byaut<strong>on</strong>omous underwater vehicleSessi<strong>on</strong>: O.19 - M<strong>on</strong>itoring 2Presentati<strong>on</strong> time: 12:20 - 12:40GJ Kirkpatrick 1 , MA Moline 2 , SELohrenz 3 , OM Schofield 41 Mote Marine Laboratory, SARASOTA,United States of America2 California Polytechnic State University,SAN LUIS OBISPO, United States ofAmerica3 University of Southern Mississippi,STENNIS SPACE CENTER, United Statesof America4 Rutgers University, NEW BRUNSWICK,United States of AmericaLaboratory and field studies havedem<strong>on</strong>strated the feasibility ofdetecting Karenia brevis blooms inthe eastern Gulf of Mexico utilizinglight absorbance spectra.Development of this technique hasbeen aimed at providing moretimely access to data andinformati<strong>on</strong> <strong>on</strong> the initiati<strong>on</strong>,transport, and effects of K. brevisblooms. Management efforts tomitigate the harmful effects ofblooms will require temporal andspatial m<strong>on</strong>itoring of phytoplankt<strong>on</strong>community tax<strong>on</strong>omic compositi<strong>on</strong>and dynamics. To achieve thistax<strong>on</strong>omic discriminati<strong>on</strong>, laboratorycultures of 12 species of microalgaerepresenting five tax<strong>on</strong>omic classeswere used to develop a library oftarget classes. A fitting routineinvolving multiple least-squaresanalyses was applied toBreveBuster absorbance spectra todetermine the ‘best fit’ estimates ofchlorophyll a c<strong>on</strong>centrati<strong>on</strong>c<strong>on</strong>tributed by each class in bothlaboratory culture mixes and naturalmixed populati<strong>on</strong>s. A ten-daydeployment of a BreveBuster <strong>on</strong> anaut<strong>on</strong>omous underwater vehicle(AUV) off the west coast of Floridain September and October 2004detected a Karenia brevispopulati<strong>on</strong> associated withcyanobacteria and diatompopulati<strong>on</strong>s which had not beenobserved by manual sampling.Multiple transects across the shelfby the AUV over this ten-day periodillustrated the spatial and temporaldynamics of the phytoplankt<strong>on</strong>community.O.19-06Retrospective GIS analyses ofthe Florida red tide databaseSessi<strong>on</strong>: O.19 - M<strong>on</strong>itoring 2Presentati<strong>on</strong> time: 12:40 - 13:00KA Steidinger 1 , JA Tustis<strong>on</strong> 2 , CA Heil 21 University of South Florida, ST.PETERSBURG, United States of America2 FL Fish & Wildlife C<strong>on</strong>servati<strong>on</strong> Comm.,ST. PETERSBURG, United States ofAmericaFlorida red tides (Karenia brevis)are the most frequent and l<strong>on</strong>gest74

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