12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006fragilis was found from June toSeptember coinciding with mucilagephenomena. Towards the end ofsummer, Gonyaulax cells wereoften found inside aggregationsalong with diatoms and heliozoans,while on many occasions only thethecae were found. Furthermore,the examination of preserved watersamples collected in 2004 revealedthat G. cf. fragilis was also presentin summer 2004 when mucilagephenomena were also intense. Thehighest abundances of Gonyaulax(7.92x10 3 cells/L) were found onAugust 2005 in integrated watersamples, while their abundance in2004 did not exceed 1.40x10 3cells/L.PO.13-32DSP shellfish toxicity in relationto occurrence of Dinophysis fortiiand D. caudata bloomsSession: PO.13 - Regional eventsŽ Nincevic Gladan, I Marasovic, SSkejic, M BužancicInstitute of Oceanography and Fisheries,SPLIT, CroatiaTemporal and spatial distribution ofDinophysis species in relation toDSP shellfish toxicity was studied atsix areas with 13 sampling stationsalong the eastern Adriatic coastfrom January 2001 to December2005. Seven potentially toxicDinophysis species were recordedincluding D. acuta, D. acuminata, D.caudata, D. fortii, D. rotundata, D.sacculus, and D. tripos. Dinophysisspecies differed in seasonaloccurrence. Dinophysis acuta, D.acuminata and D. sacculus weremore abundant in spring and autum.Dinophysis rotundata and D. triposoccurred in high abundance duringwinter and spring, while higherabundance of D. rotundata in NWAdriatic usually occurred in earlysummer. Dinophysis caudata andD. fortii showed maximumabundance in summer and autumn.Dinophysis species showed strongseasonal variability and differentspatial distribution. Diarrheticshellfish toxins outbreaks wereusually associated with thepresence of D. fortii and D. caudata.Dinophysis fortii was alwaysassociated with shellfish toxicitywhile D. caudata blooms werepresent during both periods withand without shellfish toxicity,indicating D. fortii as the most DSPtoxic species in these areas.PO.06-07The emergence and dynamics ofred tide blooms caused byCochlodinium polykrikoides inthe Peconic Estuary, NY, USASession: PO.06 - Population dynamicsR Nuzzi 1 , CJ Gobler 21 Suffolk County Department of Health Serv,YAPANK, United States of America2 Stony Brook University, SOUTHAMPTON,United States of AmericaThe dinoflagellate Cochlodiniumpolykrikoides is well known forforming harmful red tide blooms inAsian waters to the detriment offisheries and aquaculture there. Inthe US, C. polykrikoides bloomshave been less common. Here wereport on the emergence of a C.polykrikoides blooms in the PeconicEstuary, NY, USA, during the latesummer and early fall of 2004 and2005. During both years, bloomsachieved cell densities exceeding10 7 /L and chlorophyll levelsexceeding 100μg/L throughoutmuch of the estuary, althoughbloom waters were characterized byextreme patchiness. Highresolutionspatial and temporalsampling during 2005 suggested251
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006that the bloom was initiated in theupper tributaries of the PeconicEstuary in August, andprogressively spread to the outerestuary in September. Aquaculturedjuvenile oysters (Crassostreavirginica) and wild juvenile and adultsoft shell clams (Mya arenaria)experienced elevated mortalityduring the blooms, a findingparticularly troubling in light of theongoing multi-million dollar efforts torestore the Peconic Estuary’sshellfish populations. Incubationexperiments during bloom eventssuggested that both nitrogen andorganic micronutrients (vitamins)may both play a key role instimulating the growth of C.polykrikoides during bloom events.PO.05-04Nitric oxide synthase-mediatednitric oxide (NO) generation byharmful red tide phytoplankton,Chattonella marinaSession: PO.05 - Toxin analysisTatsuya Oda, Kim Daekyung, KenichiYamaguchiNagasaki University, NAGASAKI, JapanThe harmful red tide phytoplanktonspecies Chattonella marina isknown for its potent fish-killingactivity. Previous studies havedemonstrated that C. marinaproduces reactive oxygen species(ROS), and a ROS-mediatedichthyotoxic mechanism has beenpostulated. In this study, we foundthat C. marina is producingrelatively high levels of nitric oxide(NO) under normal growthconditions. We utilized achemoluminescence (CL) reactionbetween NO and luminol-H 2 O 2 todetect NO in a C. marina cellsuspension. Significant CL wasobserved in a cell-numberdependent manner, and itdecreased after addition of carboxy-PTIO, a specific NO scavenger. Theestimated level of NO produced byC. marina was higher than those ofROS. The NO generation by C.marina was also confirmed by aspectrophotometric assay based onthe measurement of the diazoreactionpositive substances (NOx)and by fluorometric assay using ahighly specific fluorescent indicatorof NO. The NO level in C. marinawas significantly reduced by L-NAME, a specific NO synthase(NOS) inhibitor, and the addition ofL-arginine resulted in an increase inNO level, whereas NaNO 2 had noeffect. These results suggest thatNOS-like enzymes are mainlyresponsible for NO generation in C.marina.PO.10-03Growth and toxin production ofthe dinoflagellate, Alexandriumminutum (Dinophyceae) isolatedfrom Tumpat Estuary,northeastern part of PeninsulaMalaysiaSession: PO.10 - Ecophysiology &autecologyT Ogata 1 , CP Leaw 1 , G Usup 2 , AKobiyama 1 , K Koike 1 , PT Lim 1,31 Kitasato University, OFUNATO CITY,Iwate, Japan2 Universiti Kebangsaan Malaysia, BANGI,MalaysiaThe tropical estuarinedinoflagelallate, A. minutum Halimwas used to determine theecophysiological adaptation inrelation to the temperatecounterparts. This species has beenfrequently associated with incidenceof paralytic shellfish poisoning(PSP) in Southeast Asia in recentyears. The effects of irradiance andtemperature on growth, nitrate252
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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