12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 20062 Japan Food Research Laboratory,NAGAYAMA, TAMA-SHI, TOKYO, JapanCapillary Electrophoresis is beingapplied in this work for the analysisof yessotoxins as an alternative tothe conventional HPLC method,which is the most commonly used.Coupling of capillary electrophoresiswith mass spectrometry (CE-MS)offers a good alternative forsensitive determination of thesetoxins and also for confirmation ofthe results obtained.CE analysis was performed, using10 mM ammonium acetate at pH8.5 as running buffer, and massspectrometric detection werecarried out through an electrosprayionisation (ESI) source. An on-linesample preconcentration approachbased on field-amplified samplestacking (FASS) was applied toincrease the sensitivity. The limit ofdetection (LOD) achieved was 0.02µg/mL, which corresponded to 1.25pg YTX loaded onto the capillary.Under these conditions, Yessotoxin(YTX) and its analogue 45-hydroxyyessotoxin(45-OHYTX) wereclearly determined by CE-MS inseveral shellfish samples as well asin marine phytoplankton cultures ofProtoceratium reticulatum.Additionally, the method wassuccessful for determination of otherlipophilic toxins present in musselsamples such as pectenotoxin-6(PTX-6). The results clearlydemonstrate the potential of CE,especially when coupled with MS,as an alternative method fordetermination of these particularcompounds.PO.13-78Establishment of cultures ofHAB organisms from theMexican Pacific coastSession: PO.13 - Regional eventsGraciela de Lara-Isassi, MCRodríguez-Palacio, ME Meave, CLozano-Ramírez, S Álvarez-HernándezUniversidad Autónoma Metropolitana-Iztapalapa, MEXICO DF, MexicoDinoflagellates are economicallyimportant in Mexico as in othertropical countries, as some speciesare toxic and can produce harmfulalgal blooms. HAB events arefrequently recorded in Mexico. Forthis reason it is important to culturethe producer organisms, which wereisolated under a microscope using amicropipette and placed individuallyinto microtiter plates. Twenty-threestrains of dinoflagellates causingred tides are maintained in ourlaboratory. Some have beenreported as toxic: Karlodiniummicrum, Scrippsiella trochoidea andGymnodinium catenatum, andothers as ichthyotoxic: Ceratiumdens, Ceratium fusus andProrocentrum micans. Clonalcultures are maintained as nonaxenic,semicontinuous, L:D 12:12,18, 20 °C ± 1 °C, illuminations of166.8 and 90.5 μmol m -2 s -1 ,respectively, using four differentculture media. Isolation of HABproducer species are vital forstudying the conditions that regulatethe growth or control the synthesisof toxic molecules, in order to createmodels to aid in early detection ofred tides events.PO.12-15Description of a novelraphidophyte species and genusfrom Delaware’s inland bays,USASession: PO.12 - Taxonomy and phylogenyE Demir 1 , Kathryn J Coyne 1 , KirkCzymmek 2 , David A Hutchins 11 University of Delaware, LEWES, UnitedStates of America160