12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.13-14Temporal and spatial distributionof Pseudo-nitzschia species(Bacillariophyceae) along the NEcoast in Catalan coastal waters,NE Spain (Mediterranean Sea)Session: PO.13 - Regional eventsS Quijano-Scheggia, E Garcés, NSampedro, J M Fortuño, K vanLenning, J CampInstituto Ciencias del Mar, BARCELONA,SpainTemporal and spatial distributions ofpotentially toxic species of thegenus Pseudo-nitzschia werestudied in 2005 along the Catalancoast (NW Mediterranean). Electronmicroscopic techniques revealedthe presence of Pseudo-nitzschiabrasiliana, P. calliantha, P.delicatissima, P. fraudulenta, P.multistriata and P. pungens. Thetwo species P. pungens and P.fraudulenta were mainly found inthe northern region, with maximumconcentrations between Februaryand May (3.2·10 4 and 5.04·10 5 cellsL -1 , respectively). Pseudo-nitzschiadelicatissima and P. calliantha werefound at the northern stationsbetween February and March(6.08·10 5 to 4.56 10 5 cells L -1 ,respectively), and appeared at thesouthern locations between Augustand November (7.92· 10 5 to3.37·10 5 cells L -1 , respectively).Pseudo-nitzschia brasiliana and P.multistriata were only occasionallydetected in the southern region, withmaximum concentrations betweenAugust and September (2.5·10 5 and4.16·10 6 cells L -1 , respectively). Thesum of the identified Pseudonitzschiaspecies showed highvalues (up to 4.16·10 6 cells L -1 )during most of the year, thepopulation initiated a sharp declineat all stations when watertemperature increased beyond 21°C(April to May). Cultured isolateswere submitted for HPLC analysisof pigments to evaluate thepossibility of monitoring naturalpopulations by a chemotaxonomicapproach.PO.05-41Emerging algal toxins in CanadaSession: PO.05 - Toxin analysisMA Quillam 1 , CM Garnett 1 , NI Lewis 1 ,R Yu 1 , W Hardstaff 1 , JM van de Riet 2 ,RA Potter 2 , WA Rourke 2 , BG Burns 21 National Research Council of Canada,HALIFAX, Canada2 Canadian Food Inspection Agency,HALIFAX, CanadaPrior to 1987, the only phycotoxinsof concern to Canada were thoseresponsible for PSP. In 1987,domoic acid caused a seriousincident of ASP in Eastern Canadaand in 1992, the toxin DTX1 wasimplicated in a DSP event in NovaScotia. In 1995, an entirely newclass of toxins, spirolides (SPX),were identified in shellfish thatcaused anomalous mouse deaths inthe lipophilic toxin assay. Followingthese incidents, a more proactiveapproach was taken, based oncomprehensive surveillance ofshellfish and plankton for other toxinclasses using LC-MS. This led tothe detection of PTX2 in net towsamples and PTX2sa in musseltissue in Nova Scotia in 2000. Morerecent discoveries includegymnodimine, DTX1 and PTX2 inBritish Columbian samples in 2003;PTX2, DTX and SPX throughoutEastern Canada; and yessotoxin(YTX) in mussels fromNewfoundland in 2004. Additionally,with the implementation of solidphase toxin tracking (SPATT), YTX,266