12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006software. The resources of allnodes will be linked so that contentfrom all local sites, as well asexternal resources accessedthrough linkouts, can be accessedfrom each node. PLANKTON*NETwill use taxonomically intelligentweb services developed by the uBioProject (http://www.ubio.org) toorganize information aboutorganisms. The modular softwarewill also be extended to containenvironmental data.PLANKTON*NET already containsmany records of harmful algae incollections from very differentgeographic areas and the coveragewill increase as new partners maketheir data collections visible throughPLANKTON*NET. By providingcomprehensive, reliable up to dateinformation it will present a valuableresource for research andmonitoring studies on harmfulalgae.PO.07-09Effects of nutrient supply ratiosand initial communitycomposition on dinoflagellatebloom formation: mesocosmstudies from the northern BalticSeaSession: PO.07 - Ecology andoceanographyA Kremp 1 , T Tamminen 2 , K Spilling 21 University of Helsinki, HANKO, Finland2 Finnish Environment Institute, HELSINKI,FinlandSince the early 1980s 'red-tides'caused by cold-waterdinoflagellates have become aregular phenomenon in the coastalN Baltic Sea, where theyincreasingly replace the typicaldiatom-dominated phytoplanktonspring bloom assemblage. Althoughthis trend has generally beenrelated to anthropogenic nutrientenrichment and changing climaticconditions, the mechanismsunderlying the relationship haveremained poorly understood. Usingmesocosms, we studied the effectsof variable nutrient additions andnutrient supply ratios on speciescomposition and dominancepatterns of natural springphytoplankton communities from thecoastal N Baltic Sea. Theexperiments which were repeatedduring 3 consecutive years showedthat bloom formation anddominance of the dinoflagellateWoloszynskia halophila primarilydepended on the size of theinoculum population and the relativeabundance of co-occurring diatoms.Variations of N/P:Si ratios did nothave any significant effect onphytoplankton development andcomposition. Addition of N and P aswell as increased irradiancegenerally stimulated the growth ofdiatoms, but could not alter theoutcome of competition betweendiatoms and dinoflagellates whenthe latter were initially dominant.Our results emphasize theimportance of efficient recruitmentstrategies and initial conditions indinoflagellate bloom formation.PO.05-10Yessotoxin profiles from culturesand planktonic field samples ofthe marine dinoflagellatesProtoceratium reticulatum andGonyaulax spiniferaSession: PO.05 - Toxin analysisB KrockAlfred-Wegener Institute for Polar and M,BREMERHAVEN, GermanyYessotoxins are a large group ofladder-frame disulfated polyethers.Yessotoxin and an array of209
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006derivatives including glycosides canaccumulate in suspension-feedingshellfish, leading to positiveresponses in the mouse bioassayfor lipophilic marine biotoxins.Yessotoxins are globally distributedin coastal and shelf waters ofdiverse locations, including Japan,Norway, Chile, New Zealand, Italyand the North Sea. There are threeknown natural sources ofyessotoxins – all are marinedinoflagellates belonging to thegonyaulacoid species Protoceratiumreticulatum, Lingoludiniumpolyedrum and Gonyaulax spinifera.Yessotoxins were originally classedas among the diarrhetic shellfishpoisoning (DSP) toxins, but they arenow regarded as a distinct group asthey do not induce diarrheagenicsymptoms. We analyzed theyessotoxin composition of P.reticulatum isolates from theBenguela Current, South Africa andcompared the profiles to cultures ofP.reticulatum and G. spinifera fromthe North Sea off the east coast ofScotland by high performance liquidchromatography coupled withtandem mass spectrometry (LC-MS/MS). The data on the culturedisolates were then compared to theyessotoxin profiles found in naturalphytoplankton assemblages fromboth locations.PO.05-01Direct selective separation ofdomoic acid by molecularlyimprinted polymersSession: PO.05 - Toxin analysisTakuya Kubo 1 , Kunimitsu Kaya 1 ,Tomoharu Sano 21 Tohoku University, SENDAI, Japan2 NIES, TSUKUBA, JapanIn the analysis of domoic acid (DA)in shellfish, phytoplankton andseawater as well as human samplessuch as blood and urine, severalmethods have been reported.However, the procedures of themethods are complicated. In orderto simplify the procedures, selectiveseparation of DA and its isomersfrom samples is the most effective.For the reason above, wedeveloped a novel selectiveseparation medium of DA using themolecular imprinting technique(MIT) that is the most effectivemethod for selective molecularrecognition. In this study, we usedan advanced method of MIT, named'fragment imprinting technique'. Todesign a template for DA, wefocused on carboxylic acid groupsin the molecule, and selectedseveral carboxylic acid groupcontainingcompounds. In the caseof o-phthalic acid as a template, theselective recognition of DA wasobserved by high performanceliquid chromatography (HPLC),while the medium prepared usingm- or p-phthalic acid as thetemplate recognized DA only to asmall extent. Finally, we achieveddirect selective separation of DAfrom shellfish-extracted sample byHPLC using o-phthalic acidimprinted polymer.PO.08-16Effects of microcystins on humanpolymorphonuclear leucocytesSession: PO.08 - ToxicologyP Kujbida, A Campa, P Colepicolo, EPinto, E HatanakaUniversidade de São Paulo, SAO PAULO,BrazilMicrocystins (MCs) are cyclicheptapeptides produced bycyanobacteria present incontaminated reservoirs. Reportedtoxic effects for microcystins are210
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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