12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006populations.High abundances of D. acuminatawere usually observed in theilluminated layer above thethermocline or at the thermoclinewhen it was within the euphoticzone and coincided with a nutricline.Dinophysis norvegica resided at thethermocline even when it was belowthe euphotic zone and even thoughno apparent increase in nutrientswas observed. Dinophysisacuminata and D. norvegicaappeared to be vertically separatedwhen co-occurring; this wasobserved even when both speciesformed maxima in the top 10 m.PO.05-24Development of an enzymelinkedimmunosorbent assay(ELISA) for detection of paralyticshellfish poisoning toxins (PSP)Session: PO.05 - Toxin analysisY Hamano, K KawatsuOsaka Pref. Inst. of Public Health, OSAKA,JapanA rapid and sensitive ELISA fordetection of PSP in shellfish wasdeveloped, using a monoclonalantibody (GT-13A), which waspreviously confirmed to reactspecifically to various kinds ofstandard PSP, and was evaluatedfor PSP monitoring. The ELISA kit,consisting of the antibody (GT-13A)solution, anti-mouse IgG-antibodycoated plate, decarbamoylsaxitoxinperoxidaseconjugate solution,washing solution and stoppingsolution, was made for practicaluse. By the ELISA kit, at least 40samples can be measured within 40min, and the detection limit of PSPwas 0.2 nmol of PSP (gonyautoxin2/3) per g of shellfish. More than1300 specimens of samples werecollected from various areas ofJapan in 2002-2004 and weremeasured by the ELISA. Goodcorrelation was observed betweenthe ELISA values and those ofmouse bioassay (MB). In theELISA, because of the highsensitivity, small amounts of PSPwere detected from 461 of 611samples which gave negativeresults by MB. The ELISA isconsidered to be a useful tool forPSP monitoring of shellfish.PO.13-29About toxic cyanobacteria inTunisia’s fresh waterSession: PO.13 - Regional eventsAsma Hamza 1 , I Zekrri 21 Institut National des Sciences et Techno,SFAX, Tunisia2 INSTM, SFAX, TunisiaA survey on cyanobacteria hasbeen conducted in aquaticenvironments in Tunisia (marinewater, dams, thermal waters, etc.).Species were named and criticalperiods identified. Speciescomposition, biomass and dynamicsof the cyanobacteria depended onmany factors. Besides the trophicstatus of a particular water mass,weather seasonality also plays animportant role. Excessive growth ofcyanobacteria and strongdominance of Microcystisaeruginosa reflected anexceptionally long hot dry period.PO.01-18Phylogeny and biogeography ofProrocentrum donghaienseSession: PO.01 - GeneticsXiaotian Han 1 , YZ Qi 2 , JZ Zou 1 , ZMYu 1 , YH Gao 3 , DD Lu 41 Institute of Oceanology, CAS, QINGDAO,China2 Institute of Hydrobiology, Jinan University,GUANGZHOU, China185
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 20063 School of Life Sciences, XiamenUniversity, Xiamen, China4 Second Institute of Oceanography, StateOceanic Administration, HANGZHOU,ChinaThis research represents the firstcomprehensive study of thephylogeny and biogeography of P.donghaiense from the East ChinaSea, Korean coast and the EastPacific (South America).The 18S ribosomal RNA gene wasused as a phylogenetic andbiogeographic indicator. The four P.donghaiense strains werepositioned i the same clade on thephylogenetic tree. The bootstrapvalue of 93.2% between the WestPacific and the East Pacific strainsindicate that the populationsoriginated from the same ancestor.Prorocentrum donghaiense strainsfrom the East China Sea andKorean coast are closely related toone another, which suggests that P.donghaiense from the East ChinaSea may be native to the Koreancoast. From the high degree ofhomogenity within the globallydistributed clade of P. donghaiense,we conclude that P. donghaiensefrom the East Pacific may be theorigin of these populations. Theclade of P. donghaiense is closelyrelated to the clade of P. minimum.PO.15-30Blooms of cyanobacteria in theBaltic Sea 1997-2006 detectedusing satellite – the phosphorusconnectionSession: PO.15 - MonitoringM Hansson, B KarlsonSMHI, Oceanographic services, VÄSTRAFRÖLUNDA, SwedenSummer blooms of nitrogen-fixingcyanobacteria are regularphenomena in the Baltic Sea. In thepast years strong and widespreadblooms have caused environmentalconcern due to nuisance, toxicityand the increased nitrogen input.The most abundant toxic species,Nodularia spumigena, can pose athreat to small animals and children.Based on satellite sensor AVHRR(Advanced Very High ResolutionRadiometer), SMHI has a compiledtime series of surfaceaccumulations of cyanobacterialblooms during the period 1997-2006. Results indicate large interannualvariations that could beconnected to periods with oxygenfree conditions in the bottom waterin the Baltic Sea and the resultingrelease of phosphorus from thesediments to deep water. Duringinflow of oxygen rich and salinewater from the Kattegat, deep watercan be moved closer to the surfaceand eventually the water with highconcentrations of phosphorus istransported into the surface layerdue to wind mixing. Since mostcyanobacteria have the ability to fixnitrogen, the main limiting factor isthe amount of availablephosphorus. Other factors that alsoinfluence the occurrence of strongblooms are the sea surfacetemperature, wind conditions andstratification.PO.05-37Analysis of phycotoxins in handpickedplankton cells by microcolumnliquid chromatographytandemmass spectrometrySession: PO.05 - Toxin analysisWR Hardstaff 1 , NI Lewis 1 , J Aasen 2 ,MA Quilliam 11 National Research Council, HALIFAX NS,Canada2 School of Veterinary Science, OSLO,NorwayThe toxin concentration and profilepresent in plankton can vary186
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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