12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006grown in areas exposed to the opensea have in general higher toxinvalues than semi-enclosed fjordsystems. There is also aconsiderable variation in toxin levelsbetween years. In 1997 musselfarmers experienced very lowlevels. In autumn 1989 to spring1990 and in early autumn 2000 toearly 2001, high levels wererecorded during 26 weeks in a row.In neither of these cases toxicitycould be linked to unusually high orlow levels of Dinophysis. The KoljeFjord region had low levels of toxinsuntil year 1998, despite regularrecordings Dinophysis in the area.Today mussels grown andharvested in this area have similartoxin levels as mussels from otherfjords in the region.PO.13-36An investigation into theecotoxicology of different strainsof Lingulodinium polyedrum fromthe Portuguese coastSession: PO.13 - Regional eventsM A ReisAlfred-Wegener Institute, HELGOLAND,GermanyLingulodinium polyedrum is knownto be a common species along thePortuguese coast. In this region thefirst record that associates L.polyedrum with red-tide eventsdates back to 1944. This species isfrequently associated with shellfishcontamination and fish mortality,and yessotoxin production hasalready been detected in L.polyedrum from other areas.Blooms occurred along the southand west coast in the summers of2004 and 2005, leading to extensiveprecautionary beach closures.However, prior to the present work,no information was availableregarding the toxicity of PortugueseL. polyedrum populations. Here, weinvestigate the potential yessotoxinproduction by different strainsisolated from the west coast andsouth coast. Toxin production wasstudied in the lag, exponential,stationary and death phase, usingHPLC-MS as a detection method.Preliminary results suggest thatnone of these strains are yessotoxinproducers and the strains are nottoxic in any of the examined growthphases. Intraspecific differences ingrowth rates were also investigatedand preliminary results will bepresented.PO.06-08Time-series study of theoccurrence of dinoflagellatecysts in surface sediments froma warm temperate region(Cascais Bay, Portugal)Session: PO.06 - Population dynamicsSS Ribeiro, A AmorimUniversity of Lisbon, LISBON, PortugalA five-year survey was conductedbetween January 2000 andNovember 2005 to assess theseasonal occurrence ofdinoflagellate cysts in surfacesediment samples from CascaisBay, Portugal. A total of 53 cystmorphotypes were found. Resultsindicate that the overall cystproduction follows a seasonalpattern, with maximum abundanceof cysts with cell contents occurringin late autumn. However, individualcyst morphotypes and dominance oftaxonomic groups present avariable year-to-year behaviour,associated with major shifts on thespecies composition of thecommunity, and some speciesshowing cyst 'blooms' at timeintervals larger than 5 years. Cysts269
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006of the toxic species Gymnodiniumcatenatum, responsible for the mainPSP events along the Portuguesecoast, and the potentially toxicspecies Lingulodinium polyedrumwere recorded, but never accountedfor significant relative abundancesduring the study period. Correlationbetween cyst assemblages andseveral environmental parameterswere studied by correspondenceanalysis. Mean SST and surfaceChl a were found to be importantfactors influencing cyst seasonality.PO.01-08Genetic variation in ribosomalDNA of Chattonella aff.verruculosa, a new harmfuldictyochophyte forming recurrentblooms in Scandinavian watersSession: PO.01 - GeneticsI Riisberg, B EdvardsenUniversity of Oslo, OSLO, NorwayThe ichthyotoxic dictyochophyteinitially named Chattonella aff.verruculosa, was for the first timerecorded in Norwegian waters in1998 and has since then formedrecurrent blooms in the North Seaand Skagerrak. We have isolatedstrains from Skagerrak (2001 and2006) and generated ribosomalDNA sequences from these strains,as well as of strains of C.verruculosa from other geographicalregions. The relationships amongthese strains and to some otherdictyochophytes were inferred frompartial LSU (D1 and D2 domains)rDNA phylogeny. The C. aff.verruculosa/C. verruculosa strainsdiverged into two well supportedclades: one clade included five C.aff. verruculosa strains fromSkagerrak and the other embracedC. verruculosa from Germany,Japan and New Zealand. Thedistance between these clades was1-2 % that may suggest aseparation at the species level. Thepartial LSU rDNA phylogenyrevealed Florenciella parvula as theclosest relative to C. aff.verruculosa/C. verruculosa. We alsoamplified the ITS1- ITS2 ribosomalDNA region by PCR. The PCRproducts were cloned, and 5 clonesfrom each of five Chattonella aff.verruculosa strains and C.verruculosa from Japan weresequenced. The ITS sequencesshowed intraspecific and intraclonalvariation and was not related togeographical origin.PO.13-52First record of a harmful bloom ofGymnodinium catenatum alongthe Michoacán coast, MéxicoSession: PO.13 - Regional eventsMónica Cristina Rodríguez-Palacio,Cruz Lozano Ramírez, Sergio AlvarezHernández, Graciela de Lara IsassiUniversidad Autonoma Metropolitana-Iztapalapa, DF, MexicoIn Lazaro Cardenas, Michoacan inNovember 2005 the first record wasmade of a harmful algal bloomcaused by the naked dinoflagellateGymnodinium catenatum Graham1943. Cochlodinium polykrikoidesMargalef 1961 was also present inthe bloom. In situ, G. catenatumformed chains of 12-64 cells, mostly56 cells, while C. polykrikoidesformed two-celled colonies only.The density of G. catenatum was560,000 cells/liter compared to20,000 of C. polykrikoides. Theformer species was the dominantdinoflagellate. Isolates of these twoPSP producers are maintained asclonal cultures, not axenic, andmaintained in a new medium270
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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