12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006has currently been sequenced andgene-finding and annotationanalysis has identified andexamined over 150 open readingframes (ORF’s). This informationhas provided an insight into thephysiology of a toxin-producingcyanobacterial bloom metagenomeand identified proteins of interestboth ecologically and in terms ofpotential in industrial processes.PO.11-03Species-specific allelopathicinteractions involving the red tidedinoflagellate Karenia brevisSession: PO.11 - AllelopathyEK Prince 1 , T Myers 1 , J Naar 2 , JKubanek 11 Georgia Institute of Technology,ATLANTA, United States of America2 University of North Carolina at Wilmingt,WILMINGTON, United States of AmericaCompetition is one of the dominantprocesses structuring marinepelagic communities. Allelopathy, amechanism of interferencecompetition by which one speciesinhibits another via excretion ofnatural products, may allow aphytoplankton species with poornutrient acquisition abilities to be adominant competitor, and may alsocontribute to the formation ofharmful algal blooms (HABs) byexcluding non-bloom-formingspecies. We have found thatallelopathy is common among Gulfof Mexico phytoplankton species,but that compounds other than wellknownHAB neurotoxins aretypically involved. Bioassay-guidedfractionation, followed byspectroscopic identification ofallelopathic compounds, is beingused to identify the molecularstructures of allelopathiccompounds produced by the redtide dinoflagellate Karenia brevis.Allelopathy may also be inducible,whereby the presence of a specificcompetitor led to increasedallelopathy by K. brevis.PO.11-04Phaeocystis globosa Scherffel,its haeomolytic and allelopathiceffectsSession: PO.11 - AllelopathyYZ Qi 1 , JS Liu 1 , WD Yang 1 , XC Peng 1 ,SH Lu 1 , Y Wang 1 , JF Chen 1 , TJ Jiang 1 ,ZH Wang 1 , YH Gao 2 , VR Marion 31 Jinan University, GUANGZHOU, China2 Xiamen University, XIAMEN, China3 University of Groningen, HAREN, TheNetherlandsPhaeocystis globosa blooms wererecorded in China since 1997. Thephysiological characteristics of thisspecies, as well as characters of itshaemolysins were described.Haemolytic substances producedunder nutrient limitation showed anallelopathic response to adverseconditions. Potential allelopathiceffects on three harmful bloomalgae, Prorocentrum donghaiense,Chattonella marina and Chattonellaovata were studied. Growth of C.marina and C. ovata was inhibitedconsiderably when co-cultured withP. globosa or cultured in cell-freespent medium. Haemolytic extractsfrom P. globosa cells in thesenescence phase had a similarinhibitory effect on the three HABspecies. The brine shrimp Artemiasalina was less affected by P.globosa. The results indicate that P.globosa has an allelopathic effecton microalgae, and this may explainits superior competitive abilities.Since addition of the haemolytictoxins from P. globosa gave similareffects on the algae compared tospent media, these compoundscould be involved in the allelopaticaction.265
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.13-14Temporal and spatial distributionof Pseudo-nitzschia species(Bacillariophyceae) along the NEcoast in Catalan coastal waters,NE Spain (Mediterranean Sea)Session: PO.13 - Regional eventsS Quijano-Scheggia, E Garcés, NSampedro, J M Fortuño, K vanLenning, J CampInstituto Ciencias del Mar, BARCELONA,SpainTemporal and spatial distributions ofpotentially toxic species of thegenus Pseudo-nitzschia werestudied in 2005 along the Catalancoast (NW Mediterranean). Electronmicroscopic techniques revealedthe presence of Pseudo-nitzschiabrasiliana, P. calliantha, P.delicatissima, P. fraudulenta, P.multistriata and P. pungens. Thetwo species P. pungens and P.fraudulenta were mainly found inthe northern region, with maximumconcentrations between Februaryand May (3.2·10 4 and 5.04·10 5 cellsL -1 , respectively). Pseudo-nitzschiadelicatissima and P. calliantha werefound at the northern stationsbetween February and March(6.08·10 5 to 4.56 10 5 cells L -1 ,respectively), and appeared at thesouthern locations between Augustand November (7.92· 10 5 to3.37·10 5 cells L -1 , respectively).Pseudo-nitzschia brasiliana and P.multistriata were only occasionallydetected in the southern region, withmaximum concentrations betweenAugust and September (2.5·10 5 and4.16·10 6 cells L -1 , respectively). Thesum of the identified Pseudonitzschiaspecies showed highvalues (up to 4.16·10 6 cells L -1 )during most of the year, thepopulation initiated a sharp declineat all stations when watertemperature increased beyond 21°C(April to May). Cultured isolateswere submitted for HPLC analysisof pigments to evaluate thepossibility of monitoring naturalpopulations by a chemotaxonomicapproach.PO.05-41Emerging algal toxins in CanadaSession: PO.05 - Toxin analysisMA Quillam 1 , CM Garnett 1 , NI Lewis 1 ,R Yu 1 , W Hardstaff 1 , JM van de Riet 2 ,RA Potter 2 , WA Rourke 2 , BG Burns 21 National Research Council of Canada,HALIFAX, Canada2 Canadian Food Inspection Agency,HALIFAX, CanadaPrior to 1987, the only phycotoxinsof concern to Canada were thoseresponsible for PSP. In 1987,domoic acid caused a seriousincident of ASP in Eastern Canadaand in 1992, the toxin DTX1 wasimplicated in a DSP event in NovaScotia. In 1995, an entirely newclass of toxins, spirolides (SPX),were identified in shellfish thatcaused anomalous mouse deaths inthe lipophilic toxin assay. Followingthese incidents, a more proactiveapproach was taken, based oncomprehensive surveillance ofshellfish and plankton for other toxinclasses using LC-MS. This led tothe detection of PTX2 in net towsamples and PTX2sa in musseltissue in Nova Scotia in 2000. Morerecent discoveries includegymnodimine, DTX1 and PTX2 inBritish Columbian samples in 2003;PTX2, DTX and SPX throughoutEastern Canada; and yessotoxin(YTX) in mussels fromNewfoundland in 2004. Additionally,with the implementation of solidphase toxin tracking (SPATT), YTX,266
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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INTERNATIONAL SOCIETY FOR THE STUDY
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12th International Conference on Harmful Algae

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