12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006The historical Florida red tidedatabase provides a cross-platformfor applying and advancing GIStechnology for the monitoring andvisualization of HABs. By using theTemporal Analyst tool, the data arebrought into an event which can beset to replay on any temporal scale;a HAB bloom can then be visualizedand tracked over time. TrackingAnalyst also creates animationloops in either a video (.avi) file oras an animated .giff. The TrackingAnalyst Data Clock Wizard can beused to create a graph of concentricrings representing months of theyear. The rings allow an illustrationto be created that represents certaintemporal patterns in the database.This is helpful in understandingwhen a event begins and ends.The data clock is able to show thatbloom events are able to carry overfrom previous years. GIStechnology is advancing rapidly tothe point that seamless merging ofdifferent databases can occuralmost near real-time. This hasapplications for monitoringprograms involving platforms, e.g.,buoys, gliders, satellites, etc. If theintent is to have web-based datamanagement and visualization, thenadvancing GIS technology willfurther this approach. End productswould be maps and animations fortime series.PO.01-11Molecular approaches for thedetection and characterization ofAlexandrium species in naturalbloomsSession: PO.01 - GeneticsKerstin Töbe, T Alpermann, U John, UTillmann, B Krock, LK Medlin, ADCembellaAlfred Wegener Institute, BREMERHAVEN,GermanyVarious molecular methods wereused to detect and discriminateAlexandrium species inenvironmental samples collectedalong the Scottish east coast in2004. Plankton samples werecollected by vertical plankton net(20 µm) hauls (20-0 m) as well asby Niskin bottle casts from discretedepths. Cells filtered ontopolycarbonate membranes wereanalysed using fluorescence in situhybridisation (FISH) in combinationwith solid-phase cytometry to detectand enumerate Alexandriumtamarense, A. ostenfeldii and A.minutum. Additionally, conventionaland real-time quantitative PCR (RTq-PCR) was applied to the samefield samples to detect, differentiateand quantify the differentAlexandrium species. For thisapproach, new species-specificprimers and MGB-probes targetingthe small- or large-ribosomalsubunit of Alexandrium spp. weredeveloped and successfully appliedin both PCR approaches. Theobtained results were comparedwith Utermöhl microscopic countsand toxin profiles generated byHPLC-FD and LC-MS/MS to detectPSP toxins and spirolides,respectively. The combined data setwas analyzed by multivariatestatistical methods to describe thevertical distribution of toxic speciesand their respective toxins profiles.PO.13-41DSP toxins in the Gulf of Finland,Baltic SeaSession: PO.13 - Regional eventsP Uronen, P KuuppoFinnish Environment Institute, HELSINKI,Finland296