12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006a triple quadrupole mass detector(API 4000), in the mode ofprecursor ion scan and product ionscan. Two isomers of trideoxyTTX,two isomers of TTX, two isomers of11-oxoTTX, anhydroTTX and traceamounts of deoxyTTX wereidentified from the toxic snailsamples. Analytical methods usingselect ion monitoring and selectreaction monitoring targeted onthese compounds were thendeveloped.PO.11-11Roles of macroalgae for HABmitigationSession: PO.11 - AllelopathySD Zhang, Y Wang, XX Song, ZM YuInstitute of Oceanology, CAS, QINGDAO,SHANDONG PROVINCE, ChinaThe effects of two macroalgalspecies, Ulva pertusa and Gracilarialemaneiformis, on the growth ofmicroalgae in co-cultured systemswere studied. Both species,especially their fresh tissues,significantly impeded the growth ofHeterosigma akashiwo, P.donghaiense and A. tamarense.Ulva pertusa affected the growth ofmicroalgae more than did G.lemaneiformis. A positive correlationbetween the initial macroalgalbiomass and their effects onmicroalgae during co-culturing wasobserved. Nutrient assays showedthat when microalgal cells weredead, nitrate and phosphate werealmost exhausted in the G.lemaneiformis co-culture, butremained at enough levels in the U.pertusa co-culture to allow forfurther growth of the microalgae.Therefore, allelopathy was theessential factor for the inhibition ofU. pertusa on the microalgae, whilethe combined roles of allelopathyand allelospoly may be responsiblefor that of G. lemaneiformis. In a coculturedsystem of G. lemaneiformiswith Scrippsiella trochoidea thesimultaneous nutrients assay alsorevealed that allelospoly might bethe main reason for inhibition of S.trochoidea under the lower initialratio of G. lemaneiformis to S.trochoidea. However, bothallelopathy and allelospoly could beresponsible for the inhibition of S.trochoidea at higher initial biomassratio.PO.09-03Sulfotransferase activity in PSPproducingAlexandrium speciesSession: PO.09 - Toxin synthesis andchemical structure of toxinsShugang Zhang 1 , Leo Lai Chan 2 , Hua-Sheng Hong 1 , Da-Zhi Wang 11 Xiamen Univeristy, XIAMEN, China2 Hong Kong University, HONG KONG,ChinaSulfotransferase (ST) is animportant modified enzyme relatedto toxin conversion in PSPproducingdinoflagellates. Thisstudy investigated ST activity in thecrude extracts of eight Alexandriumstrains with various toxincompositions. The toxinbiosynthesis pathway inAlexandrium was also examined.ST activity was only detected in thecrude extract of Alexandriumtamarense CI01 (ATCI01), whichwas able to convert GTX2/3 toC1/C2, while it was not found inother PST-producing Alexandriumstrains, even the strains with similartoxin composition as ATCI01.Moreover, ST activity was notdetected in the non-toxic A.tamarense CCMP2023. Theseresults indicated that ST is aspecies/strain-specific enzyme andthat different toxin biosynthesis311
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006pathways may exist in differentPST-producing Alexandrium strains.This requires further study at themolecular and biochemical level.PO.15-03Relationship of magnitude andposition of the algal SICF withchlorophyll-a concentrationSession: PO.15 - MonitoringDong-Zhi ZhaoNational Marine Enviroment Monitoring C,DALIAN, ChinaTo investigate the relation of suninducedchlorophyll fluorescencenear 685nm with chlorophyll-aconcentration, the reflectancespectra of red tide species such asGymnodinium sp., Heterosigmaakashiwo, Ceratium furca and otheralgae such as Nitzschia closterium,Dicrateria zhanjiangensisHu.,Platymonas sp., Chlorococcumsp., Platymonas helgolandica var.tsingtaoensis, Chlorella sp. fromfield samples and laboratorycultures was used. R max rednormalized at R560 correlated wellwith chlorophyll-a concentration(R>0.82). To different algae, thecoefficient a of the relationshipbetween R max red/R560 andchlorophyll-a presented change.The regression equation(Rmaxred/R560 =a (chl a) b) revealthat the coefficient a varied between0.037 and 1.135, b varied between0.094 and 0.727. Simultaneouslythis study established therelationship between thefluorescence peak position andchlorophyll-a concentration. Allregression coefficients were higherthan 0.75 except Chlorococcum sp.with 0.57. The position of thefluorescence peak shifted to infraredwhen chlorophyll-a concentrationincreased, but for various species,the shift velocities of peak positionswere different. Thus Heterosigmaakashiwo was the fastes and inother algae the shift was between0.1 and 0.3nm per 10mgm -3 . Noshift was observed in Dicrateriazhanjiangensis Hu andChlorococcum sp.PO.11-12The role of allelopathy in diatomand dinoflagellate blooms in theEast China SeaSession: PO.11 - AllelopathyWeihong Zhao 1 , Meimei Chen 1 ,Jiangtao Wang 21 Institute of Oceanology, CAS, QINGDAO,China2 Ocean University of China, QINGDAO,ChinaSkeletonema costatum, Kareniamikimotoi and Prorocentrumdonghaiense blooms took placesuccessively in the East China Seain the spring of 2005. Bloom waterwas collected and filtered.Skeletonema costatum, Pseudonitzschiapungens, Alexandriumtamarense, P. donghaiense, andProrocentrum micans were culturedin the above filtered solutions toexplore the role of allelopathy in thephytoplankton communitysuccession. The results showedthat the water collected from boththe S. costatum bloom and the K.mikimotoi bloom stimulated growthof the five tested species. Mixedwater from K. mikimotoi and P.donghaiense blooms stimulatedgrowth of all tested species exceptS. costatum. The stimulatory effectof K. mikimotoi on P. donghaiensewas most marked and intensifiedwith the concentration of K.mikimotoi. Thus the stimulusproduced by S. costatum on K.mikimotoi and P. donghaiense maygive it an advantage in later K.312
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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