12th International Conference on Harmful Algae

More documents

Recommendations

Info

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006During late spring and earlysummer of 2005, large scale(>10,000 km 2 ) mixed dinoflagellateblooms developed in the coastalEast China Sea, associated withmassive fish kills in coastalaquaculture areas. Samples werecollected from different stationsalong both north-south and westeasttransects during 3 cruises ofthe Chinese Ecology andOceanography of Harmful AlgalBlooms (CEOHAB) Program, fromApril-June 2005, for assessing ratesof N (NO 3 - , NH 4 + , urea, and glycine)uptake during these blooms.Nutrient uptake rates andpreferences varied during theblooms, which were explained bythe change of phytoplanktoncommunity, total nutrient availability,and its relative composition. Preandearly-bloom rates showed highuptake of nitrate, but increasingdependence on reduced nitrogensources as the blooms progressed.Later in the bloom, nitrogen uptakerates also increased withsupplemental phosphorusenrichment, suggestingphysiological phosphorus limitation.PO.01-26Genetic diversity studies onSkeletonema species(Bacillariophyta) in the coastalwaters of southern China by SSUrDNA sequence analysisSession: PO.01 - GeneticsJunrong Liang 1 , Jinfeng Chen 1 , PengWan 1 , Yahui Gao 1 , Kinchung Ho 2 , YangLi 11 Xiamen University, XIAMEN, China2 The Open University of Hong Kong,HONG KONG, ChinaSmall subunit (SSU) rDNAsequence analysis was applied tostudy genetic diversity of ten strainsof Skeletonema collected fromcoastal waters of southern China.SSU gene fragments(approximately 1666 bp long) werecloned, sequenced and analyzed forcomparison with morphologicalcharacters. Based on morphology,three species were identified: S.subsalsum (Cleve) Bethge (mainlyfrom Shenzhen water), S. marinoiSarno et Zingone (from Xiamen andHongkong waters) and S. dohrniiSarno et Kooistra (from Xiamen andHongkong waters). All strains hadpolymorphic sites, with proportionsranging from 0.1% to 1.4%.Samples of Skeletonemasubsalsum showed highersequence diversity (from 1.3% to1.4%) compared to the otherstrains. It was also found that S.subsalsum formed an individuallineage in NJ and UPGMAevolutionary trees. The strains of S.marinoi and S. dohrnii showed verylow sequence divergence (rangingfrom 0.1% to 0.3%). Thus,molecular analyses using SSUrDNA were able to clarify thegenetic diversity and taxonomiccomplexity of Skeletonema.This work was supported by NSFC(40476055) and China 973 program(2001CB409701, 2005CB422305)PO.13-81Monitoring a bloom ofPyrodinium bahamense var.compressum occurring in ElSalvador, Guatemala and Mexico(November 2005-March 2006)Session: PO.13 - Regional eventsS Licea-Duran 1 , A Navarrete 2 , RRodríguez 3 , J Bustillos 4 , B Martínez 1 , CRamírez 51 Universidad Nacional Autónoma deMéxico, MEXICO D. F., Mexico2 Universidad del Salvador, SANSALVADOR, El Salvador219
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 20063 Centro de Estudios Tecnologicos del Mar,PUERTO MADERO, Chiapas, Mexico4 Northwest Biological Research Center, LAPAZ, Mexico5 Instituto Nacional de la Pesca, MÉXICO D.F., MexicoWater samples were analyzed byinverted microscope, and saxitoxin(STX) content of dead turtles wasmeasured by HPLC methods.During the present bloom oneperson died and seven intoxicationsoccurred. In addition, 206 deaths ofturtles and numerous quantities ofjellyfish were found on theSalvadorian beaches. Data analysisfrom Sea WiFS and Modis showeda positive temperature anomaly (1-5°C) in November 2005 betweenCosta Rica and Southern Mexicolasting until March. Chlorophyll adata registered a positive anomalyas well (0.5-1.0 mg/m 3 ) following asimilar path. The concentration ofSTX in turtles ranged from 27.9 -627.8 µg STX eq/100 g, while oncollected live jellyfish 17.3 - 21.4 µgSTX eq/100 g were detected.Concentrations of STX in oysterscollected in Mexico had 58 µgeq/100 g in December 2005 and upto 200.44 µg /100 g on February2006. Microscope analysesrevealed the presence of 48,900cells ml -1 in December 2005 and aminimum of 15 cells l -1 in March2006 in Salvadorian waters; while insouthern Mexico maximum densitywas 12 cells l -1 on February 2006.Discussed also are ocean colourimagery collected during previousblooms in the same area andunpublished data obtained fromMexican coasts between December1989 to February 2002.PO.09-01New gonyautoxin analogueisolated from the toxicdinoflagellate Alexandriumminutum (Dinophyceae)Session: PO.09 - Toxin synthesis andchemical structure of toxinsPT Lim 1 , S Sato 1 , CV Thuoc 2 , PT Tu 2 ,NTM Nguyen 2 , Y Takata 1 , M Yoshida 3 ,A Kobiyama 1 , K Koike 1 , T Ogata 11 Kitasato University, OFUNATO CITY,IWATE, Japan2 Inst. of Marine Environment and Resource,HAI PHONG, Vietnam3 Prefecture Uni. of Kumamoto,KUMAMOTO, JapanSix clonal cultures of Alexandriumminutum established from HaiPhong, Northern Vietnam inOctober 2004 were examined fortoxin production. The culturescontained predominantly GTX4.Other toxin congeners GTX1 - 3,NEO and dcSTX were alsodetected. Toxin content (Qt) variedamong the strains and growthstages, and ranged from 3.0 to 12.5fmol PST cell -1 . Interestingly, a peakeluted between GTX4 and GTX1was consistently detected in all A.minutum strains with concentrationsdepending on the growth phase.The peak disappeared under nonoxidizingHPLC-FD condition butremained unchanged aftertreatment with 0.05 M ammoniumphosphate/ 10 % mercaptoethanolor 0.1 N HCl hydrolysis. The peakwas partially purified using Bio-GelP2 after GTX1 - 4 were convertedinto STXs with ammoniumphosphate /mercaptoethanoltreatment. In LCMS, mass spectraof the peak showed predominant[M+H]+ ion at m/z 396. Ion scanningfrom 150 to 450 Da showed similarfragmentation to other GTX 1-4 withparental ion of [M+H]+ at m/z396,and fragment ions [M-SO3]+ atm/z 316, and [M-SO4]+ at m/z 298.220
Page 1 and 2:
INTERNATIONAL SOCIETYFOR THE STUDY
Page 3 and 4:
Dear participantWelcome to Denmark
Page 5 and 6:
INTERNATIONAL SOCIETY FOR THE STUDY
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170: INTERNATIONAL SOCIETY FOR THE STUDY
Page 219: INTERNATIONAL SOCIETY FOR THE STUDY
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
Page 333 and 334:
Page 335 and 336:
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
show all

12th International Conference on Harmful Algae

Create successful ePaper yourself

Delete template?

Save as template?