12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006Bandtail pufferfish (S. dorsalis)muscle from Charlotte Harbor,Florida Keys, IRL, and Tequestaranged from 1 to 1778 µgSTXeq./100g tissue.PO.08-18Analysis of paralytic shellfishpoisoning (PSP) toxins frommussels obtained from theEgyptian CoastSession: PO.08 - ToxicologyAly M. A. AbdallahNat Inst. of Oceanography and Fisheries,BOKELY, ALEXANDRIA, EgyptA sensitive HPLC method fordetermination of paralytic shellfishpoisoning (PSP) based on ion-pairchromatographic separation of PSPtoxins, post-column oxidation withperiodic acid, and fluorescencedetection has been used todetermine toxin profiles ofAlexandrium minutum. Totalconcentrations of PSP were 305and 390 µg/100g mussels in Mytilusand Donax, respectively. Theconcentrations of decarbamoyltoxins in both species were muchhigher than the concentrations ofcarbomoyl toxins. In the case of thesum of STXeq, the concentration inMytilus spp. 166 µg/100g musselswas higher (three fold) than thatfound in Donax spp. (64µg/100 gmussels). The most dominant toxinamong the carbomoyl toxins in bothspecies was CTX2. This is the firstreport in the literature of PSP toxinconcentrations in mussels from theMediterranean coast nearAlexandria.PO.08-26Lack of effect of temperature onthe depuration of domoic acidfrom the king scallop PectenmaximusSession: PO.08 - ToxicologyCP Acosta 1 , C Mariño 1 , H Martín 2 , JBlanco 11 C. Invest. Mariñas, VILANOVA DEAROUSA, Spain2 Centro Tecnológico del Mar CETMAR,VIGO, SpainSome previous observations, inlaboratory and field studies, havesuggested that temperature plays aforemost role in controlling thevelocity of depuration of domoicacid from the scallop Pectenmaximus. We maintained scallopsat temperatures of 13 to 19 ºCduring ca. 75 days and checked theeffect of this factor on the domoicacid contents of four bodycompartments (digestive gland,adductor muscle, gonad andremaining tissues). No significanteffect of temperature on the finaldomoic acid content was detectedin any of the studied compartmentsnor in the total soft tissues. A slightincrease in the toxins in theadductor muscle to the total pool oftoxins was the only effect detected.PO.15-09DNA extraction method fromharmful microalgae that ispotentially applicable to an in situquantitative real-time PCRdetectionSession: PO.15 - MonitoringMasao Adachi 1 , CM Preston 2 , R MarinIII 2 , CA Scholin 21 Kochi Univ., NANKOKU, Kochi Pref.,Japan2 MBARI, MOSS LANDING, United Statesof AmericaVarious DNA extraction protocolsare in use today for quantitativereal-time PCR detection (QPCR) ofHAB species. Co-purification ofgenomic DNA and compounds thatinhibit PCR can confound analyses119
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006by producing false negativereactions. Here, we report on anew DNA extraction method forharmful microalgae that ispotentially applicable for remote insitu applications using theEnvironmental Sample Processor(ESP). A QPCR assay forHeterosigma. (Raphidophyceae)was developed using speciesspecificprimers and probe thattarget large-subunit rDNA. Filtrationtreatment with Supor (PALL, 0.22µm) or Durapore (Millipore, 0.22µm)after addition of a CH3COOKsolution post-lysis was highlyeffective at removing compoundsthat inhibit PCR. A GF/F filter wasused for the solid phase extractionof DNA after that treatment. DNAwas recovered with efficienciessimilar to that obtained using theDNeasy Plant Mini Kit (Qiagen). Astandard curve that has highlinearity was constructed withgenomic DNA extracted fromcultured Heterosigma (1cell-10,000cells) using the filter–based DNAextraction protocol. Results of thiswork suggest that this method ispotentially applicable to in situ DNAextraction and amplification systemsplaned for future developments ofthe ESP.PO.03-02The economic consequences ofred tide events on the Gulf Coastof Florida, USASession: PO.03 - Public healthCM Adams, S Larkin, R Degner, KMorganUniversity of Florida, GAINESVILLE, FL,United States of AmericaRed tide blooms (Karenia brevis)are naturally occurring events thatfrequent the Gulf of Mexico coast ofFlorida, USA. The exact causes ofsuch events, including their durationand bloom intensity, are unknown.Studies directed towardunderstanding the economic impactred tides have on coastalcommunities have only recentlybeen attempted. Two economicstudies have been completedconcerning red tide events alongthe Gulf of Mexico coast of Florida.One study attempted to assess theimpact to local business activitiesalong the southwest Florida coast.This study not only attempted tomeasure the changes in businessactivity within the region where thered tides occurred, but alsoattempted to assess the level ofawareness that local residents hadconcerning red tide events. Anotherstudy attempted to measure thechanges in business activity alongthe northern Gulf of Mexico coast ofFlorida. These studies representthe only empirical analysesaddressing the economicconsequences of red tide events inFlorida, the state which has thegreatest number of red tide eventsin the USA. Finally, an on-goingstudy is currently measuring theeconomic impact of more recent redtide events within a larger region ofthe Gulf of Mexico coast of Florida.PO.05-23A Microcystis aeruginosa bloomand the occurrence ofmicrocystins from a eutrophicfreshwater lake in Comilla,BangladeshSession: PO.05 - Toxin analysisSagir MD Ahmed 1 , Bernd Luckas 2 ,Susan Hiller 21 University of Dhaka, DHAKA, Bangladesh2 University of Jena, JENA, GermanyBangladesh is a tropical country oflarge alluvial plains with 1.3 millionfreshwater ponds and lakes. It has a120
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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