12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006(L1VASE), a modification of the L1media.PO.10-50Inorganic carbon acquistion inthree red-tide dinoflagellatesSession: PO.10 – Ecophysiology andautecologyB Rost 1 , K-U Richter 1 , N Lundholm 2 ,PJ Hansen 31 Alfred Wegener Institute for Polar andMarine Research, BREMERHAVEN,Germany2 Dept of Phycology, University ofCopenhagen, COPENHAGEN, Denmark3 Marine Biological Laboratory, Universityof Copenhagen, HELSINGØR, DenmarkCarbon acquisition wasinvestigated in marine bloomformingdinoflagellates,Prorocentrum minimum,Heterocapsa triquetra, andCeratium lineatum. Activities ofcarbonic anhydrase (CA),photosynthetic O 2 evolution, CO 2and HCO 3 - uptake rates weremeasured by membrane inlet massspectrometry in cells acclimated tolow and high pH. A secondapproach used short-term 14Cdisequilibriumincubations toestimate the carbon source utilizedby the cells. All species showednegligible extracellular CA activityin cells acclimated to low pH andonly slightly higher activities whenacclimated to high pH. Halfsaturation concentrations forphotosynthetic O 2 evolution werelow compared to RubisCO kinetics.Moreover, affinities for inorganiccarbon increased with increasingpH in the acclimation, indicating theoperation of an efficient CO 2concentrating mechanism. Rates ofHCO 3 - uptake were high in theinvestigated species, contributingmore than 80% of photosyntheticcarbon fixation. Affinity for HCO 3-and maximum uptake ratesincreased under higher pH. Modesof carbon acquisition are consistentwith the 13C-fractionation patternindicating a strong species-specificdifference in leakage. Our resultssuggest that photosynthesis inmarine dinoflagellates is not limitedby inorganic carbon even at highpH.PO.05-12Alternative bioassays for thedetection of cyanotoxinsSession: PO.05 - Toxin analysisDR RuebhartGriffith University, MEADOWBROOK,AustraliaThe principal method for detectingthe presence of unknowncyanotoxins in Australia hastraditionally been the mousebioassay. When specificcyanotoxins are suspected,instrumental methods are generallyused, but the identity of potentialcyanotoxins is not always knownand there is still the requirement fora broad screening assay for use inthe water industry. Despite thewidespread use and currentreliance on the mouse bioassay,this test has several constraints.These constraints include: lack ofprecise quantification of cyanotoxinsat low concentrations, insensitivity,difficulties in the interpretation ofresults and slow turnaround time.Additionally, animal ethicsguidelines are calling for theelimination of the use of vertebratesin applications such as toxicitytesting. This means that alternativebioassays for the detection ofcyanotoxins in water supplies need271
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006to be developed. Thus, theCombined Research Centre forWater Quality and Treatment hasprovided funding for this PhD studyto investigate, optimise and validateof a range of microbial andinvertebrate bioassays forcyanotoxin testing. The resultsrelating to the sensitivity andapplicability of these bioassays willbe presented.PO.05-21Large-scale pumping andrecovery of algal toxins from seawaterSession: PO.05 - Toxin analysisThomas Rundberget, Morten Sandvik,Chris MilesNational Veterinary Institute, OSLO,NorwayThere is an expanding need forstandard specimens of algal toxinsfor chemical analysis and toxicitytesting. DSP toxins andazaspiracids, which are producedby non-cultivable algal species, cancurrently only be harvested fromcontaminated shellfish or directlyfrom the alga when a bloom can beidentified. We have developed aportable pumping system to harvestalgae and lipophilic algal toxinsdirectly from sea water. Theautomated system can filter 600-1000 L of water per hour and be rununattended for 3-4 days. Thesystem has been evaluated withgreat success in Norway and Spainduring the 2005–2006 season withtens of milligrams of DTXs andPTXs being harvested. The materialobtained from this system is mucheasier to purify than materialextracted from shellfish, andprovides a convenient method forisolation and purification of algaltoxin standards.PO.07-10A red tide incubator in theupwelling shadow of MontereyBay, CaliforniaSession: PO.07 – Ecology andOceanographyJP Ryan 1 , AM Fischer 1 , FP Chavez 1 , RKudela 2 , P Bissett 3 , C Scholin 1 , JGower 41 MBARI, MOSS LANDING, CA, UnitedStates of America2 University of California, SANTA CRUZ,United States of America3 Florida Environmental Research Institute,TAMPA, FL, United States of America4 Institute of Ocean Sciences, SIDNEY, BC,CanadaRed tide blooms occur in coastalwaters throughout the world. Theseblooms can harm ecosystem andhuman health, and create economichardship. Due to limitedunderstanding of red tide organismsand the environmental conditionsleading to blooms, detection andmonitoring of these events ischallenging. In Monterey Bay,California, multi-platform remotesensing observations between2002-2005 indicate the existence ofa red tide 'incubator' in an upwellingshadow in a northern region of thebay. Relative to the rest of the bay,wind mixing in this region is weak,stratification is strong, andresidence times are long.Additionally, this region receivesinput from estuarine waters, whichinfluences stratification, nutrientinput, and plankton speciescomposition. Using high-resolutionairborne remote sensing fromPHILLS, AVIRIS and MAS, and timeseries of satellite observations fromSeaWiFS, MODIS and MERIS, wedetail characteristics of thisincubator region. We integrateremote sensing with observationsfrom moorings, ships, and272
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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