12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006valve gape. Bioassays wereconducted to test survival of larvalshellfish exposed to A. monilatumas whole, unconstrained cells, cellsheld in dialysis tubing, or sonicatedcells. Sonicated A. monilatumcaused a significant decrease inlarval survival, in comparison tosurvival of the control larvae thatwere tested with nontoxicAlexandrium tamarense. Overall,these data indicate that A.monilatum blooms can adverselyaffect shellfish survival by reducingclearance rate and valve gape,affecting food intake, and inducinglarval mortality.PO.13-53Early detection and intensivemonitoring during an unusualtoxic bloom of Gymnodiniumcatenatum advected into theGalician Rías (NW, Spain)Session: PO.13 - Regional eventsY Pazos 1 , A Moroño 1 , J Triñanes 2 , MDoval 1 , P Montero 1 , MG Vilarinho 3 , MTMoita 31 INTECMAR, PONTEVEDRA, Spain2 Universidad de Santiago de Compostela,SANTIAGO DE COMPOSTELA, Spain3 IPIMAR, LISBOA, PortugalCooperation between Galician andPortuguese monitoring programsallowed an early alert of aGymnodinium catenatum bloom inthe Galician Rías a month beforethe PSP toxin accumulation inmussels. Both programs detectedisolated chains of G. catenatumfrom July 2005. The Portuguesemonitoring system warned abouthigh concentrations of G. catenatumon the Lisbon shelf in Septemberand the progressive increase in theconcentrations northward thereafter.From the near real-time sea surfacetemperature fields, it was inferredthat a northward coastal currentwas present during this period.On October 24th, a strong-downwelling (Iw < -1000 m3·s-1·km 1 ) event occurred and surfacewaters warmer (>16.5 ºC) and lesssaline (35.2) flowed into the Rías,advecting a G. catenatumpopulation (max. 1.7 x10 5 cells·L -1 ).Data from a lagrangian drifter(NOAA/CoastWatch) confirmed thepresence of a northward coastalcurrent (Vel ~0.2-0.6 m·s -1 ) on thesedates. Temporal fluctuations in G.catenatum bloom dynamic followedthe upwelling-downwelling events.High cell concentrations persisteduntil December, decreasinggradually until spring 2006. After tenyears of no G. catenatum bloomingin Galician Rías, this intense andpersistent episode caused bans onmussel culture areas (averageproduction>2x10 5 tons·year -1 ) untilmid-February, having an importantsocial and economic impact.PO.10-45Carbon dioxide productionduring an exceptionaldinoflagellate bloom at TodosSantos Bay, Baja California,MéxicoSession: PO.10 - Ecophysiology &autecologyJL Peña-Manjarrez 1 , MD Martinez-Gaxiola 1 , G Gaxiola-Castro 2 , ME de laCruz-Orozco 2 , J Cepeda-Morales 21 Centro de Estudios Tecnologicos del Mar,ENSENADA, B. C., Mexico2 CICESE, ENSENADA, B. C., MexicoDuring an exceptional dinoflagellatebloom in 2005 at Todos SantosBay, Baja California (perhaps themost intense bloom during the last45 years), a 2-day survey cruisewas carried out to study the impactof this event on the CO 2 oceanatmosphereflux. Continuousmeasurements of carbon dioxide259
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006partial pressure (pC O 2 sea) alongthe sea surface were made,pCO 2 sea average values of 600?atm were measured, inducing anocean-atmosphere flux of 13.55mmol CO 2 m -2 d -1 , our estimationsrevealed that the Todos Santos Bayexported at least 97 ton CO 2 perweek to the atmosphere during thebloom. When the bloom began, thephytoplankton community wasdominated by thecatedinoflagellates (Lingulodiniumpolyedrum, Ceratium furca, C.fusus, Prorocentrum micans andDinophysis spp.) with maximum cellcounts of 7 x 10 6 cells L -1 . At theend, the dominant species were L.polyedrum together with nonthecatedinoflagellates(Gymnodinium spp., Gyrodiniumspp. and Amphidinium spp.) and thediatom Cylindrotheca closterium.Inorganic nutrients, oxygen,temperature and chlorophyll 'a'fluorescence profiles were alsomeasured. A conceptual model isproposed in which the autotrophicrespiration and bacterial oxidation oforganic matter controls thepCO 2 sea levels.PO.15-05Ribosomal DNA quantification inthe dinoflagellates Alexandriumcatenella and Alexandrium taylorifor application in real-time PCRbasedmonitoringSession: PO.15 - MonitoringA. Penna 1 , Luca Galluzzi 1 , ElenaBertozzini 2 , E Garcés 3 , MauroMagnani 11 University of Urbino, PESARO, Italy2 Università degli Studi di Urbino, PESARO,Italy3 Institut de Ciències del Mar,BARCELONA, SpainSeveral molecular methods formonitoring of HAB species havebeen recently developed. Inparticular, quantitative real-timePCR can be used for the detectionand quantification of geneticallydistinct HAB species inenvironmental samples using astandard curve as reference. Thestandard usually consists in thetarget sequences of rDNA genescloned into a plasmid, or in DNAextracted from a known amount ofcultured target cells. When aplasmid is used as standard, it isessential to know the amount of thetarget rDNA copy number per cell.In this study, the rDNA content ofthe HAB species A. catenella and A.taylori was estimated by real-timePCR using specific primersdesigned on the 5.8S rDNA region,and standard curves constructedwith serial dilutions of the clonedtarget sequences. The data wereuseful for quantification of A.catenella and A. taylori in bloomsamples using the real-time PCRbasedapproach. While A. tayloricell quantification was generally inagreement with microscope cellcount, A. catenella cellquantification was underestimatedcompared to standard countingmethod. This could be partiallyexplained by the presence of apseudogene in the 'A.tamarense/catenella/fundyense'complex. Further experiments willbe needed in order to address thisissue.260
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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INTERNATIONAL SOCIETY FOR THE STUDY
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12th International Conference on Harmful Algae

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