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12th International Conference on Harmful Algae

12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <str<strong>on</strong>g>Internati<strong>on</strong>al</str<strong>on</strong>g> <str<strong>on</strong>g>C<strong>on</strong>ference</str<strong>on</strong>g> <strong>on</strong> <strong>Harmful</strong> <strong>Algae</strong>, Copenhagen, Denmark, 4-8 September 2006(2005). Since the publicati<strong>on</strong> of thispaper the Texas coastlineexperienced a major bloom in 2005.This presentati<strong>on</strong> will dem<strong>on</strong>stratethe efficacy of the algorithm using insitu cell count data collected fromthis event. This algorithm will thenbe used to expand NOAA’s HABforecast system to include Texas.PO.10-48Intracellular phosphorusregulates alkaline phosphataseactivity of Karenia mikimotoi(Dinophyceae) and Skelet<strong>on</strong>emacostatum (Bacillariophyceae)Sessi<strong>on</strong>: PO.10 – Ecophysiology andautecologyH Yamaguchi 1 , S. Ukita 2 , M Adachi 3 , MYamaguchi 41 Kochi University, KOCHI, Japan2 Hiroshima University, HIROSHIMA, Japan3 Kochi University, KOCHI, Japan4 FEIS, Fisheries Research Agency,HIROSHIMA, JapanAlkaline phosphatase (AP) plays animportant role in utilizati<strong>on</strong> oforganic phosphorus by unicellularalgae. AP activity has beenrecognized to be regulated by theexternal phosphate c<strong>on</strong>centrati<strong>on</strong>.In the present study, however, weclearly showed that AP activity ofharmful algae Karenia mikimotoiand Skelet<strong>on</strong>ema costatum wasdirectly regulated by the intracellularphosphorus c<strong>on</strong>tent, not by theexternal phosphate. Underphosphorus-limited steady statec<strong>on</strong>diti<strong>on</strong>s in semi-c<strong>on</strong>tinuousculture, relati<strong>on</strong>ships am<strong>on</strong>g APactivity, external phosphate andintracellular phosphorus c<strong>on</strong>tent (Q)were examined. A significantnegative correlati<strong>on</strong> was foundbetween AP activity and Q of K.mikimotoi. In the case of S.costatum, AP activity increased withincreasing Q and reached amaximum of ca. 4 fmol/cell, then theactivity decreased with increasingthe Q. The threshold values of Q forinducing AP activities in bothspecies were much higher than theminimum cell quota for their growth.The results indicate that theintracellular phosphorusc<strong>on</strong>centrati<strong>on</strong> is fundamentallyimportant to understand APregulati<strong>on</strong> and organic phosphorusutilizati<strong>on</strong> of harmful algae.PO.11-09Allelopathic interacti<strong>on</strong>s betweenthe bacillariophyte Skelet<strong>on</strong>emacostatum (Greville) Cleve and theraphidophyte Heterosigmaakashiwo (Hada) Hada ex Hara etChiharaSessi<strong>on</strong>: PO.11 - AllelopathyY Yamasaki, S Nagasoe, T Matsubara,T Shikata, Y Shimasaki, Y Oshima, TH<strong>on</strong>joGraduate School, Kyushu University,FUKUOKA, JapanGrowth interacti<strong>on</strong>s betweenSkelet<strong>on</strong>ema costatum andHeterosigma akashiwo wereinvestigated by bi-algal culturesunder axenic c<strong>on</strong>diti<strong>on</strong>s. Whenthese species were inoculated athigh cell densities, growth of bothspecies was coincidentallysuppressed. In other combinati<strong>on</strong>sof inoculati<strong>on</strong> density, the speciesthat first reached the stati<strong>on</strong>aryphase seemed to be resp<strong>on</strong>sible fora remarkable decrease of the otherspecies’ density. When bothspecies were cultured without cellc<strong>on</strong>tact, growth of S. costatum andH. akashiwo were both suppressed.Furthermore, regardless of nutrientre-enrichment, filtrates from S.costatum and H. akashiwo thickcultures mutually decreased themaximum cell densities. Therefore,306

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