12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006assist in controlling blooms of toxicdinoflagellate and thus preventing abloom from being fully developed.PO.05-29Evidence of yessotoxins inAlfacs Bay - toxic effectevaluation by cell-based assaysand toxin profile determinationby liquid chromatographySession: PO.05 - Toxin analysisE Mallat 1 , E Cañete 1 , A Caillaud 1 , MFernández 1 , I Bravo 2 , B Paz 2 , JMFranco 2 , J Diogène 11 Centre d'Aqüicultura-IRTA, ST. CARLESDE LA RÀPITA, Spain2 Instituto Español de Oceanografía, VIGO,SpainIn July 2005, a bloom of thedinoflagellate Protoceratiumreticulatum at cell concentrationlevels of 1600 cells/L was detectedin Alfacs Bay, in conjunction withthe presence of Dinophysissacculus.Positive DSP mouse bioassaymeasurements indicated thepresence of diarrhetic toxins inmussels. Analytical procedureswere optimised and applied tophytoplankton and to musselextracts. Mussel samples were firstextracted in methanol/H2O (80:20),further percolated through anoctadecylsilica cartridge, derivatizedusing the dienophile fluorescencereagent, DMEQ-TAD, andsubsequently analysed by liquidchromatography coupled tofluorescence detection (LC-FD).Concentration levels of yessotoxinin mussels about 0.3 to 1.0 µg/kgwere detected during this event.Phytoplankton samples wereextracted by sonication withmethanol, followed by a clean-upstep. Okadaic acid concentrationlevels in these samples were alsostudied to confirm the toxic effectsrecorded along theProtoceratium/Dinophysis bloom.Evaluation of cell-based toxicity offield samples by estimation of cellviability, IC 50 and morphologicaleffects was studied and comparedto the analytical measurements.Protoceratium reticulatum waspreviously detected inphytoplankton samples from theEbro Delta embayments in summer2001, and concentration levels ofyessotoxin and homoyessotoxinwere then confirmed in musselsamples.PO.13-45The influence of Pseudonitzschiaaustralis blooms inshellfish domoic acidaccumulation on the Andalusiancoast (southern Spain)Session: PO.13 - Regional eventsLuz Mamán, D Jaén, R Fernández, MAOcaña, l Fernández, I MarquezLaboratorio Control Recursos Pesqueros,HUELVA, SpainDiatoms of the genus Pseudonitzschiahave been detected at theAndalusian coast since themonitoring program began in 1996.Some species from this genus areharmful because of the productionof the toxin domoic acid. During2006, samples from the monitoringprogram were analysed with LMand EM, allowing identification ofPseudo-nitzschia australis as themost abundant Pseudo-nitzschiaspecies. This event resulted inmarked toxin accumulation inseveral bivalve molluscs from theMediterranean coast.231
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.05-07Testing of a passive adsorptiondevice in the detection of DTXsunder controlled conditionsSession: PO.05 - Toxin analysisClaire Marcaillou, Florence Mondeguer,Jean Bapt Bérard, Amélie GoupilIFREMER, NANTES, FranceRecently, McKenzie and hiscollaborators described an attractivetechnique to detect lipophilic toxinsin the field. The principle is basedon the passive adsorption of toxinsonto porous synthetic resin held insmall bags. For the purpose ofusing this technique for adecontamination trial, anexperiment was set up in thelaboratory with a Prorocentrum limaculture. Prorcentrum lima is abenthic species producing DTXsand cultivable in the laboratory. Thecumulative adsorption was studiedunder three environmentalconditions: in the raw culture, in thefiltrate and in the lysate of the sameculture. The results obtained fromthis preliminary experiment were asfollows: the cumulative adsorptiondoes not seem to be modified bythe cell presence, its relationshipwith exposure time is linear overtime and for the toxin levels studied,and the reproducibility is good in allcases.PO.15-16The Environmental SampleProcessor (ESP): a robotic devicefor detecting microorganismsremotely using molecular probetechnologySession: PO.15 - MonitoringR Marin III 1 , C Scholin 1 , S Jensen 1 , BRoman 1 , J Feldman 2 , D Greenfield 1 , CPreston 1 , W Jones 1 , E Massion 1 , GDoucette 3 , T Mikulski 31 MBARI, MOSS LANDING, CA, UnitedStates of America2 Jet Propulsion Labaratory, PASADENA,CA, United States of America3 Marine Biotoxins Program, NOAA/NOS,CHARLESTON, SC, United States ofAmericaOne of our primary research goalsis to develop analytical techniquesand instrumentation that allow us touse molecular probe technology todetect microorganisms, their genesand gene products remotely, in situ.Towards that end we havedeveloped the ESP,http://www.mbari.org/microbial/ESP,a device designed to collect discretewater samples from the oceansubsurface, concentrateparticulates, and automateapplication of ribosomal RNA(rRNA) targeted DNA probe arrays(see Greenfield et al.) and antibodybaseddiagnostics (see Doucette etal.). The ESP can also be used toarchive samples for a variety ofnucleic acid analyses, microscopyand other types of analyticalprocedures after the instrument isrecovered. To date, 3 differentclasses of DNA probe arrays thattarget a variety of bacteria andarchaea, harmful algae, and larvalinvertebrates have been applied insingle field deployments inMonterey Bay, CA, lasting ~20 d. Inconcert with the probe arrays fordetecting a suite of HAB speciesthat include Pseudo-nitzschiaaustralis, P. multiseries and P.pseudodelicatissima, we havesuccessfully utilized a competitiveELISA technique for detectingdomoic acid. In this presentation wewill review the design and workingsof the ESP, and our plans for futuredeployments, developments andtechnology transfer.232
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12th International Conference on Harmful Algae

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