12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006de Recherches ISRA/Hann, DAKAR,SénégalDuring the last three decades threemassive fish kills have taken placein Senegal, however none of themhave been associated with thepresence of harmful algae. Also,there are no official records of DSP,PSP, NSP or ASP. Shellfish(bivalves) production is mainlyestuarine; the mean yield is 2,000t.y -1 locally consumed. But, newoyster and shrimp farm productionshave been set up in Saloum Estuaryduring the last two years.Freshwater fish culture is semiintensivebut new political optionsare ongoing for an industrialproduction using more intensivesystems. Production is less than100t.y -1 . Particular attention waspaid to cyanobacteria blooms inintensive Tilapia productionresearch (high phytoplanktonconcentration and their use byBrachionus plicatilis), but nonegative impact or fish mortalitiesdue to cyanotoxins have beenobserved. The growing focus onexploitation of marine andfreshwater shellfish and fish makesit relevant to monitor more detailedfor the occurrence of potentiallyharmful microalgae. In the projectESIT/CSE, receiving satelliteimages from MERIS and MODIS at250 and 1,000m resolution, theNODC team and CSE have joinedup and follow ocean colour,phytoplankton bloom and eventuallyred tides. However, small scalemonitoring in e.g. existing orplanned aquaculture sites is alsoneeded.PO.05-19LC/MS-MS determination ofparalytic shellfish poisoning(PSP) in seafood by applicationof a new hydrophilic interactionliquid chromatographic (HILIC)columnSession: PO.05 - Toxin analysisM Diener, B LuckasUniversity of Jena, JENA, GermanyParalytic shellfish poisoning (PSP)is caused by a group ofapproximately two dozen naturallyoccurring potent neurotoxins.Consequently, the development ofanalytical methods for thequalitative and quantitativedetermination of the toxinsassociated with PSP is an importanttask. In particular, the quality controlof potentially contaminated seafoodrequires exact quantification of thePSP toxins with regard tointernational regulations for publichealth protection and internationalcommerce. The broad toxicity rangeof different PSP toxins haschallenged analytical chemists todevelop accurate and reliableanalytical methods.Chemical methods for PSPdetermination are based onchromatographic separation andderivatization of the toxins prior tofluorescence detection. However,these HPLC methods complicatethe on-line coupling to a massspectrometer (MS). The newdeveloped LC/MS-MS method isbased on chromatographicseparation of the PSP toxins usinga zwitterionic-HILIC column. Thisstationary phase allows completeseparation of all relevant PSP toxinsin seafood. Low concentration ofvolatile buffer substance andabsence of ion-pair reagentsincrease the sensitivity of the MSdetection.Literature:[1] B. Luckas, L. M. Botana (ed.),Seafood and Freshwater Toxins162