12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006mussel samples (Mytiluscalifornianus) for 16 months at theSanta Cruz Wharf in Monterey Bay,we measured Dinophysisabundance in our lab and sentmussels to the regulatory FoodInspection Agency in Canada fortoxin analyses. Results indicatedthat Dinophysis average abundancewas higher (2,000 cells per Liter) insummer than in other months (90cells per Liter) and DSP toxins inmussels were higher in summer(OA+DTX-1= 0.11 µg per gram)than in other months (OA+DTX-1=0.04 µg per gram), though at leastone of the DSP toxins wasdetectable nearly year round. Asignificant correlation betweenDinophysis fortii cell biomass, thedominant biomass contributor, andOA concentrations in musselssuggests this species is the OAsource, whereas the correlationcoefficient became weaker whenthe biomass of other Dinophysisspecies was included. Thus DSPtoxins, not previously monitored onthe US west coast, mayoccasionally represent a potentialhealth threat to human consumersof mussels, and possibly other filterfeeding organisms.PO.05-13Preparation and simultaneousLC-MS analysis of fourteenshellfish toxinsSession: PO.05 - Toxin analysisM Suzuki, R Sekiguchi, M Watai, TYasumotoJapan Food Research Laboratories, TAMA,JapanFor use as calibrants in LC-MSanalysis, we prepared fourteenshellfish toxin standards: okadaicacid (OA), dinophysistoxin-1(DTX1), pectenotoxin (PTX) -1, -2, -3, -6, azaspiracid (AZA) -1, -2, -3,yessotoxin (YTX), 45-OHYTX,brevetoxin-B2 (BTXB2), 7-OpalmitoylOAand 7-OpalmitoylDTX1.The former eleventoxins were purified fromcontaminated shellfish, except forYTX which was obtained fromcultures of Protoceratiumreticulatum. 7-O-PalmitoylOA and 7-O-palmitoylDTX1 were chemicallyprepared from OA and DTX1,respectively. The purity of thestandard toxins was checked by 1H-NMR, LC-DAD, and LC-MS. Alltoxins were quantifiable in 30 min ina single run by monitoring negativeions, except for AZAs that weredetected on positive ions. Weproved the practicality of the methodby carrying out recovery tests using90% methanol extracts preparedfrom scallop and musselhepatopancreas spiked with thestandard toxins or extracts fromnaturally contaminated shellfish.PO.13-30Species dominance andpermanence of Gymnodiniumcatenatum Graham blooms onthe estern Mediterranean coast ofMorocco (1994-2004)Session: PO.13 - Regional eventsLTJ Tahri JouteiInstitut National de Recherche Halieutiq,CASABLANCA, MoroccoThe chain-forming dinoflagellateGymnodinium catenatum is themain species associated withparalytic shellfish poisoning (PSP)outbreaks in Moroccan coastalwaters, since 1994. The seasonaldistribution of G. catenatum in theMediterranean coast of Morocco(35°05’N) during the period 1994-2004 is described. Gymnodiniumcatenatum is present throughout the287