12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.05-07Testing of a passive adsorptiondevice in the detection of DTXsunder controlled conditionsSession: PO.05 - Toxin analysisClaire Marcaillou, Florence Mondeguer,Jean Bapt Bérard, Amélie GoupilIFREMER, NANTES, FranceRecently, McKenzie and hiscollaborators described an attractivetechnique to detect lipophilic toxinsin the field. The principle is basedon the passive adsorption of toxinsonto porous synthetic resin held insmall bags. For the purpose ofusing this technique for adecontamination trial, anexperiment was set up in thelaboratory with a Prorocentrum limaculture. Prorcentrum lima is abenthic species producing DTXsand cultivable in the laboratory. Thecumulative adsorption was studiedunder three environmentalconditions: in the raw culture, in thefiltrate and in the lysate of the sameculture. The results obtained fromthis preliminary experiment were asfollows: the cumulative adsorptiondoes not seem to be modified bythe cell presence, its relationshipwith exposure time is linear overtime and for the toxin levels studied,and the reproducibility is good in allcases.PO.15-16The Environmental SampleProcessor (ESP): a robotic devicefor detecting microorganismsremotely using molecular probetechnologySession: PO.15 - MonitoringR Marin III 1 , C Scholin 1 , S Jensen 1 , BRoman 1 , J Feldman 2 , D Greenfield 1 , CPreston 1 , W Jones 1 , E Massion 1 , GDoucette 3 , T Mikulski 31 MBARI, MOSS LANDING, CA, UnitedStates of America2 Jet Propulsion Labaratory, PASADENA,CA, United States of America3 Marine Biotoxins Program, NOAA/NOS,CHARLESTON, SC, United States ofAmericaOne of our primary research goalsis to develop analytical techniquesand instrumentation that allow us touse molecular probe technology todetect microorganisms, their genesand gene products remotely, in situ.Towards that end we havedeveloped the ESP,http://www.mbari.org/microbial/ESP,a device designed to collect discretewater samples from the oceansubsurface, concentrateparticulates, and automateapplication of ribosomal RNA(rRNA) targeted DNA probe arrays(see Greenfield et al.) and antibodybaseddiagnostics (see Doucette etal.). The ESP can also be used toarchive samples for a variety ofnucleic acid analyses, microscopyand other types of analyticalprocedures after the instrument isrecovered. To date, 3 differentclasses of DNA probe arrays thattarget a variety of bacteria andarchaea, harmful algae, and larvalinvertebrates have been applied insingle field deployments inMonterey Bay, CA, lasting ~20 d. Inconcert with the probe arrays fordetecting a suite of HAB speciesthat include Pseudo-nitzschiaaustralis, P. multiseries and P.pseudodelicatissima, we havesuccessfully utilized a competitiveELISA technique for detectingdomoic acid. In this presentation wewill review the design and workingsof the ESP, and our plans for futuredeployments, developments andtechnology transfer.232