12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006marine ecosystems (sea surface tomarine hydrothermal ecosystems).We used a specific oligonucleotideprobe that target the whole group IIto detect these organisms byfluorescent in situ hybridizationduring the growth of A. minutum inthe Penzé Estuary during twoconsecutive years. Members of thisgroup are efficient parasitoids ofdinoflagellates and their occurrencewas apparently tied to thetermination and demise of A.minutum blooms. Bsed on thepreferential amplification of the SSUrDNA, almost all were closelyrelated to the wellknowndinoflagellate parasitoidAmoebophrya spp. (ex ceratii).PO.06-27FINAL, an interreg program forforecasting the initiation of toxicalgal bloomsSession: PO.06 – Population dynamicsAnnie Chapelle 1 , R Raine 2 , K.Davidson 3 , C. Labry 11 IFREMER, PLOUZANÉ, France2 University of Galway, GALWAY, Ireland3 SAMS, OBAN, United KingdomIncreasing toxic events threaten theeconomy linked to aquaculture andtourism as well as human health.France, Ireland, Scotland will createa network to work on PSP eventsdue to Alexandrium and ASP eventsdue to Pseudo-nitzschia.The objectives are to investigateenvironmental factors linked to theoccurrence of toxic events and topropose an alert based on theseindicators, which can be obtainedby monitoring by weather forecastand by environmental modelling.PO.01-09Development of a molecularprobe for the harmful alga,Pyrodinium bahamense var.compressum, from Sabah,MalaysiaSession: PO.01 - GeneticsWL Chin, PL Teoh, A AntonUniversity Malaysia Sabah, KOTAKINABALU, MalaysiaHarmful algae blooms in Sabah onlyoccur in the coastal waters of westSabah, where one of the causativeorganisms is the dinoflagellate,Pyrodinium bahamense var.compressum. Pyrodinium bloomsare often unpredictable andsometimes occur at very lowdensities. One of the problems inthe monitoring and management ofthe Pyrodinium red tides is the earlydetection of the species. This studyinvolves the development of a DNAprobe from highly conserved smallsubunit rDNA regions for fast andaccurate identification of the alga.Seawater samples containingPyrodinium taken from four offshorelocations were isolated and culturedin f/2 medium. After DNA extraction,PCR amplification was carried outtargeting the rDNA of the algae. Theresults of eight restriction enzymedigestions indicated that all thecultures were similar. Sequences ofother Pyrodinium isolates were alsoobtained (Accession number:DQ500119 - 23). The size of thesequences ranged from between1580 to 1544 bp. They differed fromthe sequence of Pyrodiniumbahamense from the GenBank in 1-4 nucleotide bases.PO.12-06FITC-conjugated lectins as a toolfor differentiating betweenvarious Polynesian strains of theciguatera-causing dinoflagellate,Gambierdiscus spp.Session: PO.12 - Taxonomy and phylogeny149