12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006When a red tide occurs, filterfeedingbivalve molluscsaccumulate the toxins produced bytoxic dinoflagellates and becometoxic for human consumption.Mussels are not affected by toxinaccumulation, and when the bloomstops, they undergo a naturaldetoxification process.The detoxification speed dependson many factors, not wellunderstood so far. Among others,food availability has been proposedto play a positive role in shellfishdetoxification. Food availability formussels in a raft is affected by thetheir relative position, both in depth,because the algal population variesin the water column, and related tothe water stream, because musselsdownstream receive water whichhas been previously filtered byupstream individuals.Our objective was to investigate ifthere is a relationship between DSPdetoxification and the relativeallocation on the raft. Our resultssuggest that upstream mussels loseDSP toxicity faster. Moreover, westudied the relationship betweenfaecal contamination, coliforms andEscherichia coli, and DSPaccumulation.PO.13-87On the genus Alexandrium(Dinoflagellata) in Vietnamesewaters: - two new records of A.satoanum and A. tamutumSession: PO.13 - Regional eventsNguyen N. Lam 1 , Jacob Larsen 21 Institute of Oceanography, NHATRANG,Vietnam2 IOC Sci. & Comm. Centre on HarmfulAlgae, COPENHAGEN, DenmarkBased on observations of cellmorphology and thecal platepattern, two dinoflagellate species,Alexandrium satoanum Yuki &Fukuyo 1992 and A. tamutumMontresor, Beran and John 2004are reported here for the first timefrom Vietnamese waters.Alexandrium satoanum belongs tothe subgenus Gessnerium and ischaracterized as follows: cells widerthan long, ca. 40 µm wide and ca.32 µm long, 1’ plate not connectedto the APC and without a ventralpore. Alexandrium tamutum belongsto the subgenus Alexandrium and ischaracterized as follows:isodiametric cells, 25-30 µm, 1’connected to the APC and with aventral pore, 6' as wide as long, andsp. plate wider than long, cystmorphology unknown. With theserecords, 17 species of Alexandriumhave now been reported fromVietnamese waters.PO.08-03DNA damage and apoptosis inCHO-K1 cells following treatmentwith cylindrospermopsinSession: PO.08 - ToxicologyA Lankoff 1 , WW Carmichael 2 , JBialczyk 3 , H Lisowska 41 Swietokrzyska Academy, Institute of Biol,KIELCE, Poland2 Wright State University, DAYTON, UnitedStates of America3 Jagiellonian University, KRAKOW, Poland4 Institute of Nuclear Chemistry,WARSZAWA, PolandThe aim of our study was toexamine the impact ofcylindrospermopsin on DNAdamage in CHO-K1 cells. Thealkaline version of the comet assay,the micronucleus assay and flowcytometry were applied.The source material for theproduction of cylindrospermopsin(CYN) was a culture ofCylindrospermopsis raciborskii. Theconcentration of CYN was213
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006determined using LC/MS. CHO-K1cells were treated withcylindrospermopsin at a dose of 0.5,1 and 2 µg/ml for 24 hours. Thelevel of DNA damage as well as thefrequency of apoptosis wereassessed by the alkaline cometassay. The Olive Tail Moment wascalculated using the CASPsoftware.Micronucleus assay was used tocorrelate the results obtained withthe comet assay with thecytogenetic results. The occurrenceof early apoptosis was determinedby the Annexin test and flowcytometry.A missing correlation between thecomet assay and the micronucleusassay results may suggest thatcylindrospermopsin-induced DNAdamage observed in the cometassay may be related to the earlystages of apoptosis due to cytotoxicbut not to genotoxic action of thecompound.This work was supported by thePolish Committee for ScientificResearch No6PO5D01320PO.03-03Using beachfront restaurantsales in Southwest Florida todetermine the localized impactsof HAB eventsSession: PO.03 - Public healthSL Larkin, CM Adams, KL Morgan, RLDegnerUniversity of Florida, GAINESVILLE, UnitedStates of AmericaExtreme environmental events,such as red tide blooms, have beenidentified by owners and managersof waterfront-dependent businessesas a source of appreciableeconomic losses. For the restaurantsector in particular, these lossesmay be irrecoverable since productsare perishable and consumptioncannot be delayed entirely. In aneffort to estimate the potentialnegative correlation betweenadverse weather events and shortrunrestaurant sales, proprietarysales data are combined with redtide and tropical storm data(incidence, intensity, wind speed,and wind direction). Theobservations are on a daily basisand cover three waterfrontrestaurants in Southwest Floridafrom January 1997 throughSeptember 2005. This presentationwill illustrate the restaurant salesdata, discuss the estimated models,and describe the relationshipbetween the revenues and thevarious explanatory factorsincluding red tides and tropicalstorms. In addition, the estimatedimpacts will be used to calculate theregional impacts associated withunforeseen environmental eventsthat could aid in the development ofa cost and benefit analysis ofproposed expenditures oneducation, mitigation, research, andmonitoring.PO.10-40Growth and toxicity of thedinoflagellate Gambierdiscustoxicus under nitrogen andphosphorus limitationSession: PO.10 - Ecophysiology &autecologyJ Lartigue 1 , T Villareal 1 , RW Dickey 21 University of Texas at Austin, PORTARANSAS, TEXAS, United States ofAmerica2 Food and Drug Administration, DAUPHINISLAND, ALABAMA, United States ofAmericaCaribbean strains (CCMP 1651 and1655) of the toxic dinoflagellate214
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12th International Conference on Harmful Algae

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