12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006marine ecosystems (sea surface tomarine hydrothermal ecosystems).We used a specific oligonucleotideprobe that target the whole group IIto detect these organisms byfluorescent in situ hybridizationduring the growth of A. minutum inthe Penzé Estuary during twoconsecutive years. Members of thisgroup are efficient parasitoids ofdinoflagellates and their occurrencewas apparently tied to thetermination and demise of A.minutum blooms. Bsed on thepreferential amplification of the SSUrDNA, almost all were closelyrelated to the wellknowndinoflagellate parasitoidAmoebophrya spp. (ex ceratii).PO.06-27FINAL, an interreg program forforecasting the initiation of toxicalgal bloomsSession: PO.06 – Population dynamicsAnnie Chapelle 1 , R Raine 2 , K.Davidson 3 , C. Labry 11 IFREMER, PLOUZANÉ, France2 University of Galway, GALWAY, Ireland3 SAMS, OBAN, United KingdomIncreasing toxic events threaten theeconomy linked to aquaculture andtourism as well as human health.France, Ireland, Scotland will createa network to work on PSP eventsdue to Alexandrium and ASP eventsdue to Pseudo-nitzschia.The objectives are to investigateenvironmental factors linked to theoccurrence of toxic events and topropose an alert based on theseindicators, which can be obtainedby monitoring by weather forecastand by environmental modelling.PO.01-09Development of a molecularprobe for the harmful alga,Pyrodinium bahamense var.compressum, from Sabah,MalaysiaSession: PO.01 - GeneticsWL Chin, PL Teoh, A AntonUniversity Malaysia Sabah, KOTAKINABALU, MalaysiaHarmful algae blooms in Sabah onlyoccur in the coastal waters of westSabah, where one of the causativeorganisms is the dinoflagellate,Pyrodinium bahamense var.compressum. Pyrodinium bloomsare often unpredictable andsometimes occur at very lowdensities. One of the problems inthe monitoring and management ofthe Pyrodinium red tides is the earlydetection of the species. This studyinvolves the development of a DNAprobe from highly conserved smallsubunit rDNA regions for fast andaccurate identification of the alga.Seawater samples containingPyrodinium taken from four offshorelocations were isolated and culturedin f/2 medium. After DNA extraction,PCR amplification was carried outtargeting the rDNA of the algae. Theresults of eight restriction enzymedigestions indicated that all thecultures were similar. Sequences ofother Pyrodinium isolates were alsoobtained (Accession number:DQ500119 - 23). The size of thesequences ranged from between1580 to 1544 bp. They differed fromthe sequence of Pyrodiniumbahamense from the GenBank in 1-4 nucleotide bases.PO.12-06FITC-conjugated lectins as a toolfor differentiating betweenvarious Polynesian strains of theciguatera-causing dinoflagellate,Gambierdiscus spp.Session: PO.12 - Taxonomy and phylogeny149
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006M Chinain, O Wong, A Ung, T Darius, TRevel, P CruchetInstitut Louis Malardé, PAPEETE-TAHITI,French PolynesiaThe benthic dinoflagellateGambierdiscus spp. is the primarycausative agent of Ciguatera FishPoisoning (CFP). Currently, 6distinct species have beendescribed within this genus.Evidence for the existence of toxicand non-toxic clones within a givenspecies is also documented in theliterature. The coexistence withinnatural populations and seasonalblooms of multiple species ofGambierdiscus, comprising bothtoxic and non-toxic clones, is oftenspeculated, stressing the need for(molecular) tools capable ofdifferentiating between the variousstrains of this dinoflagellate.We compared the binding patternsof 11 FITC-conjugated commerciallectins - Dolichus biflorus (DBA),Canavalia ensiformis (Con A),Triticum vulgaris (WGA), Pisumsativum (PEA), Arachis hypogaea(PNA), Helix pomatia (HPA), Ulexeuropaeus (UEA), P.tetragonolobus (PTG), B.simplicifolia (BS-1), L. esculentum(LEA) and Lens culinaris (LcH) -between various clones of G.toxicus, G. polynesiensis, G.australes and G. pacificus isolatedfrom French Polynesia.Lectins appeared to be aninteresting tool to identify anddifferentiate between the differentspecies of Gambierdiscus.Furthermore, some lectins werealso able to discriminate betweentoxic and non-toxic clones.The potential use of the moleculesfor detection and characterization ofharmful Gambierdiscus blooms innature is discussed.PO.09-04Genetic characteristics of nontoxicsubclones obtained fromtoxic clonal culture strain ofAlexandrium tamarense(Dinophyceae)Session: PO.09 - Toxin synthesis andchemical structure of toxinsY Cho 1 , K Hiramatsu 1 , M Ogawa 1 , TOmura 2 , T Ishimaru 3 , Y Oshima 11 Tohoku University, SENDAI, Japan2 The University of Tokyo, TOKYO, Japan3 Tokyo Univ. Mar. Science & Technology,TOKYO, JapanThis study was conducted usingsubclones of the PST-producingdinoflagellate A. tamarenseobtained from an extremely rareclonal culture strain (OF935-AT6),which was found to have become amixture of toxic and nontoxic cellsduring maintenance. The nontoxiccharacteristics of UAT-014-009, anaxenic nontoxic subclone of OF935-AT6, were confirmed by threedifferent methods. Both nontoxicand toxic subclones from OF935-AT6 were confirmedphylogenetically by nucleotidesequence analysis of speciesspecificregions in rDNAs to be thecommon A. tamarense foundaround Japan. Three out of ninetoxic subclones were found to havelost toxicity during a short period oftime (four or six years), whereas theother toxic subclones retained theirtoxicity within the same range asformerly observed. The subclonesof OF-935-AT6 showed a variety ofAFLP patterns targetingmitochondrial genes (cytochrome coxidase and 5’-flanking region ofcytochrome b), whereas re-isolatedsubclones (sub-subclones) from thesubclones of OF-935-AT6 showedhomogenous toxicity and AFLPgenotypes. These results suggestthat the parent OF935-AT6 culture150
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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INTERNATIONAL SOCIETY FOR THE STUDY
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12th International Conference on Harmful Algae

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