12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006and a known volume of filteredseawater was added. Bags wereshaken for 1 min to dislodgeepiphytic microalgae. Thesuspension was preserved withneutral lugol iodine for microscopicidentification and enumeration.Epiphytic microalgae werequantified by sedimentation in asettling chamber and examinedunder an inverted epifluorescencemicroscope using calcofluorstaining. Species composition andabundance are reported. Species ofProrocentrum and Ostreopsis wereobserved, indicating the potentialrisk of toxin accumulation throughthe food web.PO.04-02Short-term feeding response ofthe mussel Mytilus chilensisexposed to diets containing thetoxic dinoflagellate AlexandriumcatenellaSession: PO.04 – Food chainsJM Navarro, AM ContrerasUniversidad Austral de Chile, VALDIVIA,ChileFrequent blooms of Alexandriumcatenella in southern Chileencouraged undertaking of thepresent study, which uses themussel Mytilus chilensis as a modelfor evaluating the feeding responseto diets containing PSP producedby A. catenella. Short-term feedingresponses were measured usingfour diets containing differentproportions of Alexandriumcatenella. Four specimens wereexposed to diets containing A.catenella and three controls werefed with a diet free of A. catenella.Diets containing the highestpercentages of A. catenellasignificantly affected clearance rateduring the initial hours. After thisperiod M. chilensis demonstrated anability to acclimate to toxincontainingdiets, approachingsimilar values to the controlmussels. The relative insensitivity ofM. chilensis to PSP resulted in therapid normalization of its feedingbehaviour, allowing it to accumulatethe paralytic toxin within a shortperiod of time. This capacity madeMytilus chilensis a good indicatorspecies for detection of PSP eventspotentially dangerous to humanhealth. The capacity for acclimationof M. chilensis may be an adaptiveproperty within the naturalpopulation of origin, which wasaffected by the toxic bloom of A.catenella during 2002.(We are grateful for financialsupport from: Grant FONDECYT1030340 and DID-UACH).PO.13-10HABs and hurricanes in FloridaSession: PO.13 - Regional eventsMerrie Neely 1 , Cynthia A Heil 1 , SueMurasko 1 , Kristy Dziemiela 1 , ErinFaltin 1 , Matt Garrett 1 , Earnest Truby 1 ,Tom Corbin 1 , Dan Carlson 2 , DaveEnglish 31 Florida Fish and Wildlife Conservation C,ST. PETERSBURG, FL, United States ofAmerica2 Florida State University, TALLAHASSEE,FL, United States of America3 University of South Florida, ST.PETERSBURG, FL, United States ofAmericaThe 2004 and 2005 hurricaneseason provided two uniqueopportunities to evaluate the effectsof post-hurricane nutrientenrichment of the West FloridaShelf (WFS) and HAB initiation andmaintenance. Multidisciplinaryresearch cruises beganapproximately 10 days after bothHurricanes Charley and Wilma, the249
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006former occurred during a periodwithout a HAB event and the latteroccurred during a HAB event. Inboth cases, nutrient loading on theWFS was up to an order ofmagnitude greater than prehurricane,wet season conditionsand much greater than dry seasonconditions. Nutrient bioassaystaken at the mouths of bays andrivers during each cruise indicatedthat nitrogen was the most limitingnutrient in these locations, althoughP co-limitation was also implicatedin some regions and light was theprimary limiting factor forphytoplankton growth in about halfthe locations. Comparisons oflimiting nutrients in similarbioassays within offshore andinshore blooms during nonhurricaneconditions providesupporting evidence of nutrientneeds in Karenia brevis blooms.The passage of Hurricanes Katrinaand Rita ameliorated a HAB relatedhypoxia/anoxia event on the WFS.PO.12-13Pseudanabaena cf. moniliformis,a new toxic cyanobacterium fromVietnamSession: PO.12 - Taxonomy and phylogenyLTT Nguyen, N Daugbjerg, Ø MoestrupBiological Institute, COPENHAGEN,DenmarkA freshwater cyanobacterium fromHuong River (Hue, Vietnam) hasbeen isolated into clonal culture(strain HOs24). Based on thegeneral morphology seen in thelight microscope (including size andshape), it was identified asPseudanabaena cf. moniliformis.However, in the transmissionelectron microscope, thin sectionsrevealed the thylakoids having aradial arrangement. It can thereforebe distinguished from thePseudanabaena group sensuKomárek and Caslavská (1991),which is characterized by a more orless concentric arrangement of thethylakoids. A detailed description ofthe ultrastructure including the cellwall and cellular inclusions is alsoprovided. The phylogeneticrelationship between this speciesand other cyanobacteria wasinvestigated by sequencedetermination of the phycocyaningene. Toxin production studies ofstrain HOs24 using ELISA andHPLC analyses showed it toproduce six variants of microcystins.PO.13-07Mucilage phenomena in NorthAegean Sea, Greece: anotherharmful effect of dinoflagellates?Session: PO.13 - Regional eventsG Nikolaidis, K. Aligizaki, K Koukaras,K MoschandreouAristotle University of Thessaloniki,THESSALONIKI, GreeceMucilage phenomena are known inthe Mediterranean Sea since the18th century, while in Greece thefirst reports go back to the ’80s.Recently, the formation of mucilagehas been associated with thedinoflagellates Gonyaulax hyalinaand G. fragilis in Tasman Bay (NewZealand) and the Adriatic Sea(Italy), respectively. Aftersuccessive mucilage formations andsubsequent fisheries problems thelast years in Greek coastal waters,samples were collected on a weeklybasis from 14 sampling stations inThermaikos Gulf (NW Aegean Sea)and sporadically from 20 sites inNorth Aegean Sea between Juneand October 2005 in order toinvestigate any causative organismfor mucilage aggregations; G. cf.250
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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