12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006PO.06-15An investigation of therelationship between Pseudonitzschiaspecies and domoicacid in Mytilus sp. in the FalEstuary, UKSession: PO.06 - Population dynamicsL Percy 1 , W Higman 2 , K Bateman 2 , EBresnan 3 , S Morris 2 , J Lewis 11 University of Westminster, LONDON,United Kingdom2 CEFAS, WEYMOUTH, United Kingdom3 Fisheries Research Services,ABERDEEN, United KingdomAn 18-month detailed time serieshas been constructed for theoccurrence of Pseudo-nitzschiaspecies and domoic acid in Mytilussp. from the Fal Estuary, UK.Pseudo-nitzschia species wereregularly observed with fourPseudo-nitzschia species isolatedinto culture; P. fraudulenta, P. cf.pseudodelicatissima, P. multiseriesand P. pungens, of which P.multiseries was confirmed toproduce domoic acid. However,domoic acid was only detected inshellfish during a peak in Pseudonitzschia,when TEM analysisconfirmed the dominant species tobe P. cf. pseudodelicatissima.Spatial and temporal data indicatedthat these cells entered the estuaryfrom the seaward source (i.e. theEnglish Channel). There were alsoperiods where Pseudo-nitzschiaspecies occurred without thepresence of domoic acid in shellfish.The presence of a range of nondomoic acid producing Pseudonitzschiastrains/species in the FalEstuary could explain noncorrelation between cell abundanceand domoic acid concentration inshellfish in this region. In addition,the use of size classing of Pseudonitzschiacells provided limitedcapability in aiding speciesprediction and therefore hasrestricted capability in the predictionof domoic acid accumulation inshellfish in this region.PO.08-06Cyanobacterial toxins as triggersfor oxidative stress in plantsSession: PO.08 - ToxicologyAnja Peuthert, Stephan PflugmacherIGB, BERLIN, GermanyCyanobacterial toxins have beenshown to have adverse effects onplants, both terrestrial and aquatic.Microcystins are cyclicheptapeptides and the main groupof cyanotoxins. During exposure ofplants to cyanobacterial toxins theformation of reactive oxygenspecies can occur. These reactiveoxygen species have strongreactivity and are able to interactwith other cellular compounds likelipids, proteins and DNA.Plants do have an effectiveantioxidative system which will limitthe negative effects caused byreactive oxygen species. Seedlingsof Alfalfa exposed to purifiedcyanobacterial toxins and cell-freecyanobacterial crude extractshowed uptake of toxin, andadverse effects on germination,growth and root development.Antioxidative enzymes, such assuperoxide dismutase (SOD) andcatalase were examined at theprotein- as well as on the genelevel.PO.13-63First evidence of spirolideaccumulation in northwesternAdriatic shellfishSession: PO.13 - Regional eventsS Pigozzi 1 , M Cangini 1 , A Ceredi 1 , FMagnani 1 , A Milandri 1 , M Pompei 1 , E261
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006Riccardi 1 , L Bianchi 2 , L Boschetti 2 , SMontanari 2 , S Rubini 21 Centro Ricerche Marine, CESENATICO(FC), Italy2 IZS Lombardia-Emilia Romagna,FERRARA, ItalyIn autumn 2003, shellfish samplesfrom the Northwestern Adriatic Sea,collected during the routinemonitoring programme, testedpositive by mouse bioassay forlipophilic toxins (Yasumoto, 2001).Unusual symptoms and theextremely short survival times (aslow as 5 min), alerted the monitoringoperators, suggesting the presenceof compounds previouslyundetected in Italy. During theevent, the dinoflagellateAlexandrium ostenfeldii reached itsmaximum concentration of 15,612cells/L. The microalga, isolated andgrown in culture, was demonstratedto produce mainly spirolide 13-desmethyl C but no PSP toxins(Ciminiello, 2006). Here we reporton the first evidence of spirolideaccumulation in Adriatic mussels.Analyses were carried out by liquidchromatography tandem massspectrometry.PO.05-34Use of electrospray tandem massspectrometry for identification ofmicrocystins during acyanobacterial bloom eventSession: PO.05 - Toxin analysisE Pinto 1 , HV Frias 1 , P Colepicolo 1 ,KHM Cardozo 1 , MA Mendes 1 , VMCarvalho 2 , D Tomazela 31 Universidade de São Paulo, SÃO PAULO,Brazil2 Instituto Fleury, SAO PAULO, Brazil3 Waters Tecnologies, SAO PAULO, BrazilDrastic environmental conditionssuch as elevated temperature,abrupt pH variation, low turbulence,and high nutrient inputs canenhance the development of toxiccyanobacterial blooms in lakes andreservoirs. This study describes theoccurrence of four microcystinvariants (MC) in a bloom in theeutrophic reservoir Billings, in SaoPaulo City. The bloom sample wascollected in October 2003, andMicrocystis was the main genusfound. The MC were separated andpurified by reverse phase highperformance liquid chromatography(RP-HPLC). Their structures wereelucidated by electrospray ionizationtandem mass spectrometry (ESI-MS/MS) and four variants weredetermined: MC-RR, MC-LR, MC-YR, and MC-hRhR. MC-hRhR isdescribed for the first time as a newvariant of MC with twohomoarginines at positions 2 and 4in its structure. ESI-MS/MS analysisthus provides a powerful andconvenient tool for thedetermination of variants of MC.These results represent animportant contribution to theknowledge of the biochemistry oftoxic cyanobacteria and their toxins,specifically in Sao Paulo State.PO.04-03Effects of harmful algae on rotiferfeeding behaviour andreproduction: Karenia brevisuses chemical defense to detergrazersSession: PO.04 – Food chainsC Pirkle, TW Snell, J KubanekGeorgia Institute of Technology, ATLANTA,United States of AmericaBloom-forming phytoplankton mayavoid becoming prey forzooplankton because phytoplanktoncells are toxic, unpalatable, ornutritionally inadequate to supportzooplankton growth or reproduction.Using laboratory feeding assays, we262
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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