12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006Fish and wildlife mortalitiesassociated with the 2005 Floridared tideSession: PO.13 - Regional eventsLJ Flewelling 1 , Cindy Heil 1 , JayAbbott 1 , Karen Atwood 1 , AprilGranholm 1 , Sheila O Dea 1 , AllenFoley 1 , Deborah Fauquier 2 , HowardBrown 1 , Sentiel Rommel 1 , AlexCostidis 1 , Danielle Stanek 1 , Michellevan Deventer 3 , Gabriel Vargo 3 , JanLandsberg 11 FL Fish & Wildlife Conservation Comm.,ST. PETERSBURG, FL, United States ofAmerica2 Mote Marine Laboratory, SARASOTA,FL, USA3 University of South Florida, College ofMarine Science, ST. PETERSBURG, FL,USAThroughout 2005, the Florida Gulfcoast experienced one of the mostsevere Karenia brevis red tideevents in recent decades withsignificant impacts on aquaticanimals and benthic systems. Theearliest mortalities began withoffshore fish kills in January. In earlyMarch, mortalities of the Floridamanatee increased, followed in thesummer by wide-scale strandings ofbottlenose dolphin and multiplespecies of sea turtles. By August, alarge-scale bloom-relatedhypoxic/anoxic zone developedoffshore of southwest Florida,affecting hundreds of square milesof patch reefs and other benthiccommunities. Divers reportedwidespread benthic mortalities,including corals, multipleinvertebrate and vertebrate taxaand multiple reef fish species.Large numbers of aquatic birdswere affected throughout the bloomperiod.The temporal sequence of themortalities reflects movement of thebloom over time as well as theecology of the affected animals androutes of toxin exposure. Analysesdemonstrated the accumulation ofbrevetoxins in the food web, withexposure confirmed in multipleanimal species. With the exceptionof the fish kills and benthicmortalities associated with thehypoxic zone, ingestion is believedto be the primary lethal route ofexposure in these events.PO.05-15First report of the production ofspirolides by Alexandriumperuvianum (Dinophyceae) fromthe Mediterranean SeaSession: PO.05 - Toxin analysisJM Franco 1 , B Paz 1 , P Riobo 1 , GPizarro 2 , R Figueroa 2 , S Fraga 2 , IBravo 21 Instituto de Investigaciones Marinas,VIGO, Spain2 Instituto Español de Oceanografía, VIGO,SpainFour strains of Alexandriumperuvianum obtained from restingcysts in the western Mediterraneanin 2002 and 2004 were analyzed forthe presence of toxins. No PSPtoxins were detected by LC-FD afterpostcolumn derivatization.Nevertheless, spirolides weredetected by LC-MS in the fourstrains. The major componentswere 13-desmethyl spirolide C (m/z692,5) with 90% of the total toxincontent, followed by spirolide B(m/z 694,5) (6%) and smallquantities of spirolide D (m/z 708,5)(2,7%) and 13-desmethyl spirolideD (m/z 694,5) (2%). Also traces ofspirolide C (m/z 706,5) were found.This is the first report of theproduction of spirolides by thisspecies. Given the greatmorphological similarity between A.peruvianum and A. ostenfeldii, inwhich the main difference is theshape of the sulcal anterior plate,174