12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006limit of 1 mg/kg shellfish meat, notexceeding 1.2 mg/kg. A prolongedpersistence of YTXs was observedin all three areas studied. At AveiroLagoon it was possible to associatetemporary increases in YTX’scontamination with the presence inthe plankton of Protoceratium spp.and Gonyaulax spinifera, but notwith Lingulodinium polyedrum. Thisis the first report of contamination ofbivalve molluscs from Portugal withYTXs.PO.15-33Minimizing economical losseswith 'real-time' HAB surveillanceSession: PO.15 - MonitoringE Granéli, C EsplundKalmar University, KALMAR, SwedenCyanobacterial blooms coveringalmost the entire Baltic Sea is anormal feature in July-August. Forthe tourism industry at the island ofÖland, SE Sweden, the economicallosses during the summer of 2005amounted to16-21 million euros. Asremote sense satellite images havea low resolution, from the pictures ofthe blooms it looks like that allÖland beaches are covered withdecomposing algae. In reality, theseblooms rarely affect the westernside of the island. By having anintensive daily real-time surveillanceof the algal accumulation on thebeaches, with quick transfer (withinhours) of this information to thepublic, it is possible to get thetourists back, showing that onalmost all days of the two months ofblooms, most beaches are fit forbathing. The 'real-time' monitoringis be done by volunteers(pensioners, youth, camp-siteworkers, etc.) who get thenecessary training at the Universityof Kalmar. Among the skills to belearned by the volunteers areharmful algal species identificationand quantification, analyses ofcyanobacterial pigments,temperature, salinity, etc. Theresults for the pilot study (July-August) will be presented.PO.10-31Using quantification of geneexpression to investigate theinitiation phase and dynamics ofAlexandrium catenella blooms(Dinophyceae)Session: PO.10 - Ecophysiology &autecologyD Grzebyk 1 , MS Shin 1 , E Masseret 1 , MLaabir 1 , A Pastoureaud 2 , Y Collos 1 , AVaquer 11 University Montpellier 2, MONTPELLIER,France2 IFREMER, LER/LR, SÈTE, FranceInitiation is a critical phase ofharmful algal blooms that has beenoverlooked in field investigationscarried out for understanding andpredicting their occurrence, for tworeasons. First, when toxic cells aredetected in phytoplankton byconventional monitoring methods,their concentration is alreadyrelatively high (ca 100 cells/L) andenvironmental conditions are likelychanging with respect to theinitiation conditions that triggeredthe cell proliferation. Second, whena cell is observed, its physiologicalstatus remains unknown: is this cellactually in growth phase? To getcloser to the initiation period ofHAB, new tools are required whichare more sensitive, specific, andable to give information on cellactivity and proliferation dynamics.In the framework of our programinvestigating Alexandrium catenellablooms in the Thau Lagoon (FrenchMediterranean), we are developingmolecular assays to analyze geneexpression (mRNA quantity per cell)181
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006using reverse transcription andquantitative PCR. We have selectedtwo target genes coding for theRubisCO enzyme (performingcarbon fixation and reflecting cellmetabolic activity) and the PCNA(proliferating cell nuclear antigen)protein involved in cell division. Totest these assays, we will presentvariations of gene expressionanalyzed in various experimentalconditions.PO.06-22Effect of selenium on PseudonitzschiaseriataSession: PO.06 - Population dynamicsIC Guimarães Nogueira 1 , VMVasconcelos 1 , P Vale 21 Ciimar, PORTO, Portugal2 IPIMAR-Instituto de Investigação das Pes,LISBOA, PortugalThe laboratory culture of toxigenicpennate diatoms of the genusPseudo-nitzschia often gives lowergrowth rates. This is inadequate forecophysiological studies and forstudies involving acid domoicquantification or web-transfer. It issuggested in the literature thataddition of extra silicon andselenium is beneficial for growth ofdiatom cultures, such as Pseudonitzschiasp. In this work weevaluated the effect of selenium onPseudo-nitzschia seriata growthrates and also on f/2 mediumfeatures such as pH, salinity andconductivity, over 15 days at 10 ºC.Addition of selenium, atconcentration of 1.29 mg Na 2 SeO 3/L, caused a slight increase of thegrowth rate and pH. In contrast,significant differences were found inconductivity and salinity betweenculture mediums with and withoutselenium. No domoic acidproduction was detected by HPLC.PO.14-05Flow cytometry in conjunctionwith dual staining assessesviability of Microcystis cells afterexposure to bacteriaSession: PO.14 - MitigationJR Gumbo, TE CloeteUniversity of Pretoria, PRETORIA, SouthAfricaFlow cytometric (FCM) techniquewas used to assess viability ofMicrocystis cells after exposure toBacillus mycoides B16. The FCManalysis involved assessment oftwo cellular functions, esteraseactivity and membrane integrity,after dual staining with Fluoresceindiacetate (FDA) and Propidiumiodide (PI). The dual staining ofMicrocystis cells revealed differentcell populations: living, membranecompromised and dead cells. Theresults show that after 2 h ofincubation with B. mycoides B16 thepopulation of dead Microcystis cellswas 12%, membrane-compromisedcells was 80% and live cells was8%. The demography of Microcystiscells changed after 6 d ofincubation, with population of deadMicrocystis cells was 51%, thosewith membranecompromised cellswas 34% and live cells was 15%. Inbacteria treated samples theMicrocystis biomass was reducedby over 95% whereas with thecontrol samples the biomassincreased by over 1000%. Thebiocontrol agent B mycoides B16appeared to cause Microcystis celllysis resulting in cells withcompromised membranes with aSEM micrographs signatureequivalent to samples that havebeen lysed with copper sulphate, aknown algicide. B. mycoides B16could therefore be considered as a182
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INTERNATIONAL SOCIETYFOR THE STUDY
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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