12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006(42 600 cells of Dinophysis spp/L),[AO] was 25,4 pg/cell, while forDinophysis acuta, the [DTX2] was25 pg of equivalent AO/cell.than 32 psu. The characteristicsand the distribution of thispotentially toxic genus arediscussed.PO.13-64A post-tsunami study on thediversity of dinoflagellates in thecoastal area of Phang-ngaProvince, ThailandSession: PO.13 - Regional eventsN Mongkonsangsuree, C Songroop, APiumsomboon, N PhapavasitChulalongkorn University, BANGKOK,ThailandAn assessment of tsunami damageon the mangrove and coastalecosystem of Nam Khem Village,Phang-nga province, southernThailand was carried out in October2005. The area was damaged bythe tsunami event in December2004. The water intrusion intomangrove creeks and thedestruction of mangrove trees bythe over-washed wave causedserious problems for both theecosystem diversity and function.Thus, a study on the diversity anddistribution of phytoplankton andbenthic microalgae was conductedto assess the long-term impact ofthe tsunami. Duplicate watersamples were collected fromsubsurface and bottom depths at 7stations located along the waterchannel between the main land andKho-kao Island and in the mangrovecreeks. A total of 15 genera ofdinoflagellates were found in thearea with Protoperidinium, Ceratiumand Prorocentrum as the mostabundant genera. Dinoflagellatecysts were also observed. A highdensity of Prorocentrum, more than200 cells/l, was recorded in the midchanneland the adjacent mangrovecreek where the salinity was higherPO.01-07Molecular characterization andmorphological variability ofseven strains of thedinoflagellate ProrocentrumminimumSession: PO.01 - GeneticsM Monti, B CatalettoOGS, TRIESTE, ItalyThe class Dinophyceae has beenthe subject of several molecularstudies that have clarified thephylogenetic position ofdinoflagellates with respect to otherprotists and have helped toestablish new genera. However,within the same dinoflagellatespecies, morphological features cansometimes vary in response tochanging environmental conditionsor physiological states.Prorocentrum minimum has been asubject of interest to taxonomistsbecause of its highly variablecellular morphology. This has givenrise to a debate about whether thedifferent shapes represent separatespecies or only one variable taxon.The morphological diversity ofseven strains of P. minimum fromdifferent geographical areas wasinvestigated using a range of cellcharacters, and intraindividualgenetic diversity was assessed bysequencing the internal transcribedspacer regions (ITS1 – ITS2). Threestrains originated from the AdriaticSea, two from the Baltic Sea, onefrom the Gulf of Finland and onefrom Chesapeake Bay (USA). Allstrains were kept under controlledconditions, at their original salinities.From the morphological and243
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006phylogenetic analyses, it wasconcluded that size and shapevariations are indicative of differentmorphotypes of the same species.PO.16-10Life cycle transformations in HABspecies: Pseudo-nitzschia in theGulf of NaplesSession: PO.16 – Life cyclesM Montresor, D D'Alelio, SMMcDonald, D Sarno, A ZingoneStazione Zoologica 'Anton Dohrn', NAPOLI,ItalyThe EU project SEED(http://www.icm.csic.es/bio/projects/seed/) aims at improving andextending our knowledge of theshifts between the different lifestages in HAB species and atidentifying the environmental andphysiological factors that regulatethose transitions. The two domoicacid-producing diatoms Pseudonitzschiagalaxiae and P.multistriata regularly bloom in theGulf of Naples (Mediterranean Sea).The former species consists ofthree genetically distinct populationswith different seasonal timing oftheir bloom, whereas the latterspecies generally blooms in latesummer-autumn, with a secondarypeak in early winter. The temporaldynamics of their cell size spectrummay provide a hint for theoccurrence of sexual reproduction.Do the different species havedistinct environmental windows foroptimal growth? Does a bloomrepresent the occasion in whichsexual reproduction occurs? Whereare the species when they are notseen in plankton? We providepreliminary data gained fromlaboratory experiments and in situobservations of P. galaxiae and P.multistriata in the Gulf of Naples.PO.08-13Lipid, fatty acid and sterolcomposition of 8 species ofKareniaceae: chemotaxonomyand putative lipid phycotoxinsSession: PO.08 - ToxicologyBD Mooney 2 , PD Nichols 2GM Hallegraeff 1 ,1 University of Tasmania, HOBART,Australia2 CSIRO Marine and AtmosphericResearch, HOBART, AustraliaLipid class, fatty acid and sterolcomposition of 8 species ofichthyotoxic marine gymnodinioiddinoflagellates (Karenia,Karlodinium,Takayama) wasexamined. The commondinoflagellate polyunsaturated fattyacids (PUFA), octadecapentaenoicacid (OPA 18:5ω3) anddocosahexaenoic acid (DHA22:6ω3), were present in all speciesin varying amounts (14-35% and 8-23%, respectively). The very longchainPUFA (VLC-PUFA) 28:7ω6and 28:8ω3 were present at lowlevels (
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12th International Conference on Harmful Algae

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