12th International Conference on Harmful Algae

More documents

Recommendations

Info

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006mussel samples (Mytiluscalifornianus) for 16 months at theSanta Cruz Wharf in Monterey Bay,we measured Dinophysisabundance in our lab and sentmussels to the regulatory FoodInspection Agency in Canada fortoxin analyses. Results indicatedthat Dinophysis average abundancewas higher (2,000 cells per Liter) insummer than in other months (90cells per Liter) and DSP toxins inmussels were higher in summer(OA+DTX-1= 0.11 µg per gram)than in other months (OA+DTX-1=0.04 µg per gram), though at leastone of the DSP toxins wasdetectable nearly year round. Asignificant correlation betweenDinophysis fortii cell biomass, thedominant biomass contributor, andOA concentrations in musselssuggests this species is the OAsource, whereas the correlationcoefficient became weaker whenthe biomass of other Dinophysisspecies was included. Thus DSPtoxins, not previously monitored onthe US west coast, mayoccasionally represent a potentialhealth threat to human consumersof mussels, and possibly other filterfeeding organisms.PO.05-13Preparation and simultaneousLC-MS analysis of fourteenshellfish toxinsSession: PO.05 - Toxin analysisM Suzuki, R Sekiguchi, M Watai, TYasumotoJapan Food Research Laboratories, TAMA,JapanFor use as calibrants in LC-MSanalysis, we prepared fourteenshellfish toxin standards: okadaicacid (OA), dinophysistoxin-1(DTX1), pectenotoxin (PTX) -1, -2, -3, -6, azaspiracid (AZA) -1, -2, -3,yessotoxin (YTX), 45-OHYTX,brevetoxin-B2 (BTXB2), 7-OpalmitoylOAand 7-OpalmitoylDTX1.The former eleventoxins were purified fromcontaminated shellfish, except forYTX which was obtained fromcultures of Protoceratiumreticulatum. 7-O-PalmitoylOA and 7-O-palmitoylDTX1 were chemicallyprepared from OA and DTX1,respectively. The purity of thestandard toxins was checked by 1H-NMR, LC-DAD, and LC-MS. Alltoxins were quantifiable in 30 min ina single run by monitoring negativeions, except for AZAs that weredetected on positive ions. Weproved the practicality of the methodby carrying out recovery tests using90% methanol extracts preparedfrom scallop and musselhepatopancreas spiked with thestandard toxins or extracts fromnaturally contaminated shellfish.PO.13-30Species dominance andpermanence of Gymnodiniumcatenatum Graham blooms onthe estern Mediterranean coast ofMorocco (1994-2004)Session: PO.13 - Regional eventsLTJ Tahri JouteiInstitut National de Recherche Halieutiq,CASABLANCA, MoroccoThe chain-forming dinoflagellateGymnodinium catenatum is themain species associated withparalytic shellfish poisoning (PSP)outbreaks in Moroccan coastalwaters, since 1994. The seasonaldistribution of G. catenatum in theMediterranean coast of Morocco(35°05’N) during the period 1994-2004 is described. Gymnodiniumcatenatum is present throughout the287
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006year, but maximum concentrationsare detected in spring (early May)and autumn (November). PSPtoxicity was detected in shellfishafter winter and autumn blooms.Alexandrium minutum, also a PSPagent, was observed in spring andsummer. Dinophysis caudata, aDSP producer, bloomedoccasionally in spring, and thepotential ASP producer Pseudonitzschiaspp. increased to bloomproportions sometimes in winter. Ahigh correlation between toxic andnon-toxic species has beenestablished (r>0, 7); Lauderia spp.and Thalassionema nitzschioideswith G. catenatum; Ceratium sp withDinophysis spp; Navicula andCeratium karstenii with Alexandriumspp; Coscinodiscus andLeptocylindrus minimus withPseudo-nitzschia spp.PO.05-16On the correlation betweenMMPB and ELISA methods fortotal microcystin concentrationsSession: PO.05 - Toxin analysisH Takagi 1 , T Sano 2 , K Kaya 31 National Inst. for Envirometal studies,TSUKUBA, Japan2 National Inst. for Environmetal Studies,TSUKUBA, Japan3 Tohoku University, SENDAI, JapanTotal microcystin concentrations ofcultured cells and naturalwaterblooms were determined bythe methods of MMPB and ELISA.In the case of ELISA, the crossreactivityof the antibody andmicrocystin depended on the type ofmicrocystin present. The crossreactivityof microcystin-LR and RRwas almost the same, whereasthose of other variants were lowerthan that of microcystin-LR. Whentotal microcystin concentrations ofnatural waterblooms weredetermined by the two methods, theresults obtained from the MMPBmethod were always higher thanthose from the ELISA method.However, in some samples theresults obtained from the ELISAmethod were higher than those fromthe MMPB method. These resultssuggest that some non-microcystincompounds reacted with themicrocystin antibody, or theantibody was denatured bybiosurfactants in the fractions of thecell extracts.In the fractionation of naturalwaterblooms using HPLC, thecross-reactivity was found in nonmicrocystinfractionations. If thefractions did not contain even atrace amount of microcystin, nonmicrocystincompounds ought tohave reacted with the antibody. Toconfirm this hypothesis, compoundsin the non-microcystin fractionswere isolated and purified by HPLCand thin-layer chromatography.PO.14-13Growth control of toxicmicroalgae cell by using directcurrent electricity, direct currenthigh voltage electrical discharge,ozone gas dissolution andhydrogen peroxideSession: PO.14 - MitigationShin Takano, Asami Touno, HitoshiOgawaUniv. Tamagawa, Machida-shi, TOKYO,JapanThe direct current electricity iscalled DC (direct current), and hasbeen used for portable apparatuseslike batteries, flashlights, etc. Theelectric current in a solution showselectro-physical phenomena likethe electrical short circuit throughthe surface. The DC high voltagedischarge in water will find the288
Page 1 and 2:
INTERNATIONAL SOCIETYFOR THE STUDY
Page 3 and 4:
Dear participantWelcome to Denmark
Page 5 and 6:
INTERNATIONAL SOCIETY FOR THE STUDY
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238: INTERNATIONAL SOCIETY FOR THE STUDY
Page 287: INTERNATIONAL SOCIETY FOR THE STUDY
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
show all

12th International Conference on Harmful Algae

Create successful ePaper yourself

Delete template?

Save as template?