ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Calling and Scrutinizing Allelic Discrimination Data
About the Allelic
Discrimination View
5-10 End-Point Analysis
The SDS software graphs the results of allelic discrimination runs on a scatter plot
contrasting reporter dye fluorescence. After signal normalization and multicomponent
analysis, the software graphs the normalized data from each well as a single data
point on the plot.
The following figure illustrates the components of the Allelic Discrimination plot.
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The following table describes the elements of an SDS plate document:
Component Description
1 Marker drop-down list Determines the marker data that the software displays
within the plot.
2 Call drop-down list When a datapoint is selected, this menu allows you to
assign an allele call to the datapoint within the scatter plot.
3 Toolbar Contains the following tools for manipulating the plot.
Icon Description
Selects individual data points by clicking or groups of datapoints by clicking
and dragging a box across a group of data points.
Selects groups of datapoints by encircling them with the tool.
Repositions the view within the plot by clicking and dragging the screen.
Zooms the plot by clicking the mouse button within the plot or by clicking
and dragging a section of the plot to view.
Zooms out on the plot by clicking the mouse button within the plot.
4 Scatter plot A scatter plot of data points from the run.
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