ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Preparing and
Running the
Pure Dye Plate
To prepare a plate document for the pure dye run: (continued)
Step Action
8 Save the Pure Dye plate document as follows:
a. From the File menu, select Save.
b. From the Files of type drop-down list, select ABI PRISM SDS Single Plate (*.sds).
c. From the Save dialog, click the File name text field, and choose from the
following:
Plate Format Type…
384-Well PureDye_
For example, the file name for a plate run on May 31, 2001
would be: PureDye_053101.
96-Well PureDye_Plate_
d. Click Save.
For example, the file name for a Pure Dye Plate 1 run on
May 31, 2001 would be: PureDye_Plate1_053101.
The software saves the plate document.
9 Prepare and run the pure dye plate as explained below.
IMPORTANT A background run must be performed prior to running a pure dye plate. See
“Performing a Background Run” on page 7-13 for more information.
To prepare the pure dye run:
Step Action
1 Briefly centrifuge the pure dye plate.
2 Load the pure dye plate into the 7900HT instrument as follows:
a. From the plate document in the SDS software, click the Instrument tab.
b. From the Real-Time tab in the Instrument tabbed page, click Open/Close.
The instrument tray rotates to the OUT position.
c. Place the pure dye plate into the instrument tray.
Note TheA1positionislocatedinthetop-leftsideoftheinstrumenttray.
3 Click Start.
The 7900HT instrument begins the pure dye run. The method for a pure dye run is
hard-coded into the software and consists of a single 2-min hold at 60 °C.
Note Before starting the run, the instrument may pause (up to 15 min) to heat the
heated cover to the appropriate temperature.
4 When the pure dye run is complete and the Run Complete dialog box appears:
a. Click OK to close the dialog box.
b. Click Open/Close, and remove the pure dye plate from the instrument tray.
c. Extract the pure dye calibration information as explained on page 7-20.
System Maintenance 7-19