ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Stopping the
Sequence Detector
from the
SDS Software
4-26 Run Setup and Basic Operation
IMPORTANT Read the following directions carefully before stopping a run-in-progress.
Run Type Action
End-Point Runs From the Plate-Read tab of the plate document, click Stop.
♦ Allelic
Discrimination
Real-Time Runs
♦ Absolute
Quantification
♦ Melting Curve
Has the Cover reading in the Real-Time tab reached 104 °C?
♦ NO (the heated cover temperature is below 104 °C)
From the Real-Time tab of the plate document, click Stop.
♦ YES (the heated cover temperature has reached 104 °C)
The instrument has begun to run the plate and is in the process of
thermal cycling. Determine a course of action from the options in
the following table:
Reason for Stopping the Run Then…
Forgot to make a change to the plate
document such as adding a detector,
detector task, or sample name
(not including mistakes to the
temperature profile)
♦ Forgot to add a reagent to the plate
(suchasenzymeormastermix)
♦ Programmed the plate document
with the wrong thermal profile
do not stop the run.
Allow the instrument complete the run, then edit
the plate document before analyzing the data.
The software does not use detector or sample
information until the plate document is analyzed.
a. From the Real-Time tab of the plate
document, click Stop.
b. Determinehowfarintotheruntheinstrument
has progressed.
c. Based on the state of the run, determine
whether the plate can be re-run.
d. If necessary, eject the plate and add the
missing reaction component.
e. If desired, re-run the plate by re-creating the
plate document.