ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Extracting the
Background
7-16 System Maintenance
In this procedure you will extract the calibration values from the background plate
document. Once extracted, the SDS software stores the data as part of the calibration
file located in the Calibration subdirectory of the SDS 2.0 directory.
To extract the background component from the run data:
Step Action
1 From the Analysis menu, select Extract Background.
The software attempts to extract the background signal and displays the success of
the extraction in a dialog box.
Dialog Box Then...
the run is successful
2 From the File menu, select Save.
The software saves the plate document.
3 From the File menu, select Close.
The software closes the plate document.
The raw spectra read from
theBackgroundplate
conform to acceptable limits.
Proceed to step 3.
the run is unsuccessful.
The software has stopped
the extraction because one
or more raw spectra exceed
2500 FSU.
Troubleshoot and decontaminate the sample block as explained in “Background Runs” on
page 8-8.