ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Analysis Checklist
WhereYouArein
the Procedure
6-20 Real-Time Analysis
The following checklist illustrates your current position in the overall procedure:
Done Step Procedure See Page
✓ 1 Create an absolute quantification plate document. 4-6
✓ 2a a. Create detectors for the absolute quantification probes. 4-7
b. Copy the detectors to the plate document. 4-8
✓ 3a a. Configure the plate document with detector tasks
(NTC, Standard, and Unknown).
4-11
b. Assign quantities to the wells of the plate document that
contain standards.
4-12
✓ 4 a. Program the method for the absolute quantification run. 4-13
b. Add a temperature ramp to the thermal profile. 4-16
✓ 5 Choose from the following:
If running… Then…
a single plate continue to step 7.
the first plate in a series Save the plate document as an ABI PRISM
of plates with identical SDS Template Document as explained on
assay configurations page 4-17.
✓ 6 Create a plate document from the template created in
step 5.
✓ 7 Configure the document with sample names and plate
information.
a. Steps 2 and 3 can be eliminated by importing the plate document setup information from a tab-delimited
text file. See “Importing Plate Document Setup Table Files” on page B-2 for more information.
4-18
4-19
✓ 8 Prepare and run the dissociation curve plate or plates. 4-20
9 Analyze the run data. 6-21
10 View the results of the dissociation curve analysis. 6-22
11 Determine melting temperature (Tm ) values for the derivative
peaks.
12 Choose from the following post-analysis options:
♦ Reanalyze the run data.
♦ Adjust the display settings for the results table, plate grid,
and plate document plots.
♦ Print elements of the plate document.
♦ Export the plate document results table or plots.
6-23
6-24