ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

A(continued)
Automation Controller software
about 2-14, 4-34
adding plates to the plate queue 4-35
configuring for operation 4-37
ejecting a plate 4-38
launching 4-34
monitoring instrument progress 4-38
removing plates from the plate queue 4-35
starting the plate queue 4-38
stopping the plate queue 4-38
B
background run 7-13 to 7-16
about 7-13
constructing a background plate 7-15
creating a plate document 7-14
extracting 7-16
preparing a background plate 7-15
troubleshooting 8-8
when to perform 7-13
bar code information
entering into a plate document 3-19, 4-19
bar code readers
fixed position, See fixed-position bar code reader
hand held, See hand-held bar code reader
baseline
about A-10
configuring value for automatic analysis 6-8
setting manually 6-10
beam splitter
See instrument, optics system
Block readout (from the Real Time tab) 4-25
bold text, convention 3-4
C
calibrating the 7900HT instrument
adjusting the plate-sensor switch 7-28
aligning the fixed-position bar code reader 7-40
aligning the plate handler 7-32
performing background run 7-13
performing pure dye (spectral) run 7-17
CAUTION attention word, definition 1-2
changing
gripper finger pads 7-43
pane, view, and plot sizes 3-15
sample block module 7-4
charged coupled device array
See instrument, optics system
checklists
absolute quantification 6-7
allelic discrimination 5-8
dissociation curve 6-20
plate document setup
absolute quantification 4-3
allelic discrimination 4-4
dissociation curve 4-4
chemical safety 1-3
Index-2
chemistry
non-optimized 8-5
SYBR Green A-3
TaqMan A-2
troubleshooting 8-4 to 8-7
cleaning
gripper finger pads 7-43
sample block wells 7-12
closing
instrument tray
from the Automation Controller software 4-38
from the SDS software 4-27
plate documents 3-14
Zymark Twister software 7-39
comments
adding to a detector 4-7
adding to a plate document 4-19
computer
about 2-7
hard drive partitions 2-7
maintaining 7-46
minimum system requirements 2-7
troubleshooting 8-14
turning ON 3-5
configuring the 7900 instrument for 9600 emulation 4-14
consumables
384-Well Optical Reaction Plates D-3
96-Well Optical Reaction Plates D-3
improper or damaged plastics 8-7
Optical Adhesive Covers D-3
Optical Cap Strips D-3
writing on reaction plates 8-6
contamination
decontaminating the sample block 7-11
fluorescent, common sources 8-6
isolating on the sample block module 8-8
contextual menus, using 3-20
copying
detectors to a plate document 4-8
markers to a plate document 4-10
Cover readout (from the Real Time tab) 4-25
creating
detectors 4-7
markers 4-9
plate documents 3-12, 4-6
for running background plates 7-14
for running custom pure dyes 7-22
for running pure dye plates 7-18
from a template 4-18, 4-32
C T ,Seethreshold cycle
custom pure dyes
adding to the pure dye set 7-21 to 7-23
creating a custom pure dye plate 7-21
customer support, See technical support
cycle set, adding to a method 4-15