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ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

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<strong>7900HT</strong> Instrument<br />

Internal<br />

Components<br />

Automated Plate<br />

Handling <strong>System</strong><br />

Heated Clamp<br />

2-4 Product Overview<br />

The <strong>7900HT</strong> instrument contains the hardware used for thermal cycling and detection<br />

of fluorescent chemistries (see page 2-2). The figure below illustrates the major<br />

subcomponents of the instrument described in further detail below.<br />

! CAUTION Do not remove the cover to the <strong>ABI</strong> PRISM <strong>7900HT</strong> <strong>Sequence</strong> <strong>Detection</strong><br />

<strong>System</strong>. Only a qualified Applied Biosystems service engineer may repair or adjust the internal<br />

components of the <strong>7900HT</strong> instrument. Failure to comply can result in serious injury and/or<br />

damage to the instrument.<br />

! WARNING PHYSICAL HAZARD. Keep hands and loose clothing away from the instrument<br />

tray and door at all times during instrument operation. The <strong>7900HT</strong> instrument contains several<br />

internal components that can cause serious physical injury.<br />

The <strong>7900HT</strong> instrument features an automated plate handling system to provide easy<br />

loading and removal of plates from the instrument. In combination with the automation<br />

module, the plate handling system allows unattended operation of the instrument.<br />

!<br />

WARNING PHYSICAL INJURY HAZARD. Hot Surface. Use care when working around this<br />

area to avoid being burned by hot components.<br />

The <strong>7900HT</strong> instrument features a heated clamp that provides optimal heat transfer<br />

and uniform heating during thermal cycling. When the instrument tray loads a plate,<br />

the clamp applies a downward pressure of 70 lbs (31.8 kg) onto the consumable.<br />

During the run, the clamp maintains a constant temperature of 105 °C (±3°C) to<br />

prevent condensation within the consumable wells.<br />

GR2112<br />

Laser<br />

CCD<br />

camera<br />

Reader<br />

Tray<br />

Door<br />

Optics<br />

system<br />

Heated clamp<br />

Automated plate<br />

handling system<br />

Sample block<br />

module

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