ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

T(continued)
TaqMan RNase P Instrument Verification Plates
about 7-24
analyzing 7-26
kits D-4
preparing a plate document 7-25
running 7-24, 7-26
tasks, See detector tasks
technical support F-1
templates
about 3-11
creating a single plate document 4-18
creating multiple plate documents 4-32
saving as 4-17
thermal cycler block, See sample block module
thermal cycling protocol, See methods
threshold
about A-10
configuring for automatic analysis 6-8
improper setting 8-4
setting manually 6-11
threshold cycle
calculation A-10
relationship to PCR product A-11
Time readout (from the Real Time tab) 4-25
Time Remaining readout (from the Real Time tab) 4-25
T m ,Seemelting temperature
troubleshooting 8-2 to 8-17
7900HT Instrument 8-14
background runs 8-8
chemistry problems 8-4 to 8-7
computer 8-14
end-point runs 8-13
fixed-position bar code reader 8-17
pure dye runs 8-10
real-time runs 8-11
SDS software 8-14
Zymark Twister Microplate Handler 8-17
turning ON the ABI PRISM 7900HT Sequence Detection
System 3-5
U
upgrading
operating system software 7-47
SDS software 7-48
user guide
conventions 3-4
organization of
terminology 3-3
V
viewing, well information 3-16
views
hiding 3-16
maximizing/minimizing 3-15
resizing 3-15
showing 3-16
visualizing
outliers 6-12
W
WARNING attention word, definition 1-2
warranty statement G-1
well inspector 3-22
Y
Y Inter readout (from the Standard Curve Plot) 6-14
Z
zooming
allelic discrimination plot 5-11
plate grid wells 3-18
Zymark Twister Microplate Handler
See plate handler
Zymark Twister software
about 2-14
aligning the plate handler 7-32 to 7-39
closing 7-39
launching 7-32
testing the plate sensor switch 7-30
Index-7