- Page 1 and 2: ABI PRISM ® 7900HT Sequence Detect
- Page 3 and 4: © Copyright 2001, Applied Biosyste
- Page 5 and 6: iv Section:PlateDocumentSetup......
- Page 7 and 8: vi A Theory of Operation Fluorescen
- Page 9 and 10: Attention Words and Warning Labels
- Page 11 and 12: About Waste Profiles A waste profil
- Page 13: Safe Instrument Use Before Operatin
- Page 17 and 18: System Overview About the 7900HT In
- Page 19 and 20: 7900HT Instrument Internal Componen
- Page 21 and 22: Optics System IMPORTANT Do not remo
- Page 23 and 24: Bar Code Readers 2-8 Product Overvi
- Page 25 and 26: Compatible Consumables Consumables
- Page 27 and 28: 2-12 Product Overview
- Page 29 and 30: Sequence Detection System Software
- Page 31 and 32: Working with SDS Software Files Fil
- Page 33 and 34: Getting Started Before You Begin If
- Page 35 and 36: Conventions Used in This Manual How
- Page 37 and 38: Reading the Instrument Status Light
- Page 39 and 40: About the Software Interface 3-8 Ge
- Page 41 and 42: About the Status Bar The status bar
- Page 43 and 44: 3-12 Getting Started Exercise 1: Cr
- Page 45 and 46: 3-14 Getting Started Exercise 5: Cl
- Page 47 and 48: Lesson 3: Using the Plate Grid 3-16
- Page 49 and 50: 3-18 Getting Started Exercise 3: Zo
- Page 51 and 52: Lesson 5: Using Contextual Menus Le
- Page 53 and 54: Tabbed Pages Plate documents can co
- Page 55 and 56: Before You Begin Background Informa
- Page 57 and 58: Allelic Discrimination Checklist Di
- Page 59 and 60: Step 1 - Creating a Plate Document
- Page 61 and 62: Copying and Applying Detectors to t
- Page 63 and 64: Copying and Applying Markers to aPl
- Page 65 and 66:
Assigning Quantities to Standards f
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Programming Methods for Allelic Dis
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Programming a Temperature Ramp for
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Step 6 - Creating a Plate Document
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Step 8 - Running the Plate on the 7
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Saving the Plate Document Saving th
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Running the Plate To begin the sing
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Stopping the Sequence Detector from
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Disconnecting the Software from the
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Adding Plate Documents to the Plate
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Creating Plate Documents Using the
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Running Plates Using the Automation
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Loading Plates onto the Automation
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Operating the 7900HT Instrument Usi
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End-Point Runs on the 7900HT Instru
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Overview Allelic Discrimination on
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5-6 End-Point Analysis Genotypic Se
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Analysis Checklist WhereYouArein th
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Calling and Scrutinizing Allelic Di
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5-12 End-Point Analysis To call all
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5-14 End-Point Analysis To analyze
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Real-Time Analysis 6 In This Chapte
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Section: Absolute Quantification In
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Algorithmic Manipulation of Raw Dat
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Analysis Checklist WhereYouArein th
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Analyzing the Run Once you have con
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To set the baseline and threshold v
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Viewing Results Viewing the Analysi
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After the Analysis Changing the Pla
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Section: Dissociation Curve Analysi
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Example Results The following figur
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Analyzing the Run Data Analyzing th
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Determining T m Values The followin
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System Maintenance 7 In This Chapte
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Section: Maintaining the 7900HT Ins
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Handling the Sample Block Removing
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Replacing the Sample Block IMPORTAN
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Changing the 7900HT Plate Adapter W
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Decontaminating the Sample Block Wh
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Performing a Background Run When to
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Preparing and Running a Background
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Performing a Pure Dye Run When to P
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Preparing and Running the Pure Dye
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Adding Custom Dyes to the Pure Dye
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To create a template for running th
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Materials Required The following ma
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Section: Maintaining the Plate Hand
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To adjust the plate sensor switch:
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To test the sensitivity of the plat
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Aligning Input Stack 1 (Zymark Posi
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Aligning the Plate Handler to the I
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Defining the Bottom of the Stack To
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To define the positions of the rema
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To position the fixed-position bar
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Cleaning and Replacing Gripper Fing
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Section: Maintaining the Computer a
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Defragmenting the Hard Drive Upgrad
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Troubleshooting 8 In This Chapter T
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Table 8-1 Troubleshooting Table (co
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Imprecise Pipetting The calculated
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Contaminated Sample Block Improper
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To determine the location of the co
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Real-Time Runs (Quantitative PCR an
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End-Point Runs (Allelic Discriminat
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Troubleshooting Software and Comput
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Zymark Twister Microplate Handler a
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Theory of Operation A In This Appen
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Basics of SYBR Green Chemistry The
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Mathematical Transformations Overvi
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Real-Time Data Analysis Kinetic Ana
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Fluorescence vs. Amplified Product
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Significance of Threshold Cycles Be
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Importing Plate Document Setup Tabl
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Setup Table File Format Example Set
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Setup Table Elements (continued) Nu
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Exporting Graphics Exporting a Plot
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B-10 Importing and Exporting Plate
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Assay Development Guidelines TaqMan
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Order Reagents Note Because part nu
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Design Tips for Quantitative PCR As
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Interchangeable Sample Block Module
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Instrument Maintenance and Verifica
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References E Bloch, W. 1991. A bioc
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Limited Warranty Statement G G Warr
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Index Symbols >symbol 3-4 Numerics
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D DANGER attention word, definition
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P panes hiding 3-16 maximizing/mini
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T(continued) TaqMan RNase P Instrum
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ABI PRISM ® 7900HT Sequence Detect