ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

3-12 Getting Started
Exercise 1: Creating a Plate Document
You will need to create a plate document for every plate you run on the 7900HT
instrument. The following procedure explains how to create a plate document using
the SDS software.
To create a plate document:
Step Action
1 If not already active, launch the SDS software as explained on page 3-7.
The SDS software workspace appears.
2 Choose one of the following options:
♦ Click the New Document button ( ) from the General toolbar, or
♦ From the File menu, select New.
The New Document dialog appears.
IMPORTANT The SDS software can handle multiple documents simultaneously,
however the processing speed of your computer will decrease with each open
document. For that reason, Applied Biosystems recommends limiting the number of
open documents to 10.
3 Configure the New Document dialog box with the following settings:
Drop-Down List Select…
Assay Absolute Quantification
Container 384 Wells Clear Plate
Template Blank Template
4 Click OK.
The software displays a new plate document with appropriate attributes.
Exercise 2: Saving a Plate Document
The Save command stores any changes to the plate document setup information and
display settings. The following procedure explains how to save the open plate
document.
To save the plate document:
Step Action
1 Choose one of the following options:
♦ Click the Save button ( ) from the General toolbar, or
♦ From the File menu, select Save As.
2 From the File of type drop-down list, select ABI PRISM SDS Single Plate (*.sds).
3 Click the File name text field, and type Practice.
4 Click Save.
The software saves the plate document to a file entitled Practice.sds.
Note Do not close the plate document at this time.