ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

To determine the location of the contamination on the sample block: (continued)
Step Action
Example: (continued)
b. The raw data from
the selected wells
does not contain
the peak.
The contaminated
well must be in the
last four wells of
columns 7-12.
c. The raw data from
the selected wells
contains the peak.
The contaminated
well must be in
the last four wells
of columns 10-12.
d. The raw data from
the selected wells
contains the peak.
The contaminated
well must be in
the last two wells
of columns 10-12.
e. By selecting each
of the wells from
the last two wells
of columns 10-12,
thelocationofthe
contaminated
well (G10) is
determined.
6 Repeat step 4 until you identify the location of each contaminated well.
7 Decontaminate the sample block as explained in “Decontaminating the Sample
Block” on page 7-11.
8 Run a background plate to confirm that the contaminants have been removed.
If the contamination is present after running the background plate for a second time,
the background plate is likely to be the source of contamination.
Troubleshooting 8-9