ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Step 1 – Creating a Plate Document
About ABI PRISM
Plate Documents
Creating a Plate
Document
4-6 Run Setup and Basic Operation
Every plate run on the 7900HT instrument requires the creation of a plate document
within the SDS software. Plate documents are virtual representations of the
consumables (MicroAmp® 384/96-well plates) used to contain samples and reagents
during runs.
Plate documents contain the following information:
♦ Detector information and arrangement on the plate
♦ Marker information and arrangement on the plate (allelic discrimination only)
♦ Sample information and their arrangement on the plate
♦ Method parameters for the run (absolute quantification only)
To create a plate document:
Step Action
1 If not already open, launch the SDS software as explained on page 4-5.
2 Choose from the following:
♦ From the File menu, select New, or
♦ From the tool bar, click the New Document button ( ).
3 Configure the New Document dialog box with settings for the run as follows:
Drop-Down List Select…
Assay the type of assay appropriate for your plate.
Note If performing a dissociation curve experiment, select
Container
Absolute Quantification from the Assay drop-down list.
the type of plate you intend to run.
Template Blank Template
Assay drop-down list
Container drop-down list
Template drop-down list
Barcode text field
(Leave blank)
Note Because this chapter emphasizes the use of a template file, the Barcode
text field is left blank. However, if creating a plate document to run a single plate,
scan or type the bar code for the plate into the Barcode text field.
4 Click OK.
The software displays a new plate document with the appropriate attributes.
5 Create and copy detectors (and markers) to the new plate document as described
on page 4-7.