ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

System Overview
About the 7900HT
Instrument
Supported Runs and
Chemistries
2-2 Product Overview
The ABI PRISM® 7900HT Sequence Detection System is a second-generation
sequence detection system instrument designed for automated, high-throughput
detection of fluorescent PCR-related chemistries. The instrument is capable of
real-time, end-point, and dissociation curve analysis of assays arrayed on multiple
formats. The 7900HT instrument is optimized for use with Applied Biosystems
chemistries including those related to nucleic acid quantification and detection.
The ABI PRISM 7900HT Sequence Detection System is designed to support several
PCR-related chemistries available from Applied Biosystems and affiliated companies.
The SDS software features two types of runs to support the variety of applications.
Run/Analysis Description Supported Chemistries
End Point
(Plate Read)
In an end-point run, the 7900HT instrument
collects a single reading after completing a
PCR run. After analysis by the SDS software,
the resulting multicomponent data is used to
assess the presence of target sequences in
the unknown samples.
Real Time In an real-time run, the 7900HT instrument
collects data during each cycle of a
pre-programmed PCR run. After analysis by
the SDS software, the resulting threshold
cycle values (C T) are used to establish
quantitative relationships between the initial
template concentrations of the unknowns
and those of the controls.
♦ Allelic Discrimination
♦ Absolute Quantification
♦ Dissociation Curve
Analysisa a. For dissociation curve analysis, the instrument collects data during a pre-programmed temperature ramp
and plots the negative of the first derivative for normalized fluorescence over time.
Note For detailed information on any of the chemistries supported by the ABI PRISM 7900HT
Sequence Detection System, see the appropriate chapter for the type of run (see Chapter 5,
“End-Point Analysis,” and Chapter 6, “Real-Time Analysis,” respectively).