ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Before You Begin
Background
Information
Getting More
Information from
the Online Help
Maximizing
Throughput for
End-Point Runs
4-2 Run Setup and Basic Operation
Chapters 5 and 6 include brief discussions of the experiments that can be performed
using the 7900HT instrument. Before beginning, you may want to review the
appropriate chapter for your experiment:
Run Type Experiment See Page
End-point Allelic Discrimination 5-3
Real-time Absolute Quantification 6-3
Dissociation Curve Analysis 6-17
The SDS software features an online help system that can guide you through the
procedures for setting up, performing, and analyzing runs. To get help at any time,
click the button located within the dialog box or window in which you are working.
For end-point applications such as allelic discrimination, the throughput of the
ABI PRISM® 7900HT Sequence Detection System can be increased by dividing the
workload between the 7900HT instrument and several thermal cyclers. Unlike
real-time runs, the 7900HT instrument collects data for end-point runs after the
completion of the PCR. Consequently, the thermal cycling of end-point plates can be
done elsewhere and then transferred to the 7900HT instrument afterwards for data
collection and analysis.
IMPORTANT To perform the thermal cycling and the plate read using the 7900HT instrument,
run the plate first as a real-time plate document and then again as an allelic discrimination plate
document (see “Step 4 – Programming the Plate Document Method” on page 4-13 for the
procedure).