ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Prepare a
Background Plate
Document
7-14 System Maintenance
To prepare a plate document for the background run:
Step Action
1 Launch the SDS software.
2 From the File menu, select New.
The New Document dialog appears.
3 Configure the New Document dialog box as follows:
Drop-Down List Select
Assay Background
Container
Template Blank Template
4 If the background plate is labelled with a bar code, click the Barcode text field and
scan the bar code number using the hand-held bar code reader.
5 Click OK.
The software creates a plate document with the attributes for a background run.
Note Do not modify the background plate document. The method for a
Background run is hard-coded into the SDS software and consists of a single hold
at 60 °C for 2 min. Because the plate contains only deionized water, the plate
document does not require sample or detector labels.
6 Save the plate document as follows:
a. From the File menu, select Save.
The Save dialog appears.
b. Click the File name text field, and type Background_.
For example, the file name for a background plate run on May 31, 2001 would
be: Background_053101
c. Click Save.
The software saves the plate document. The software is now configured for the
Background run.
7 Prepare and run the plate as explained on page 7-15.