ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Preparing and
Running a
Background Plate
To conduct the background run:
Step Action
1 Choose from the following:
If using… Then…
a background plate from a
Spectral Calibration Kit
an ABI PRISM Optical
Reaction Plate
2 Briefly centrifuge the background plate.
3 Load the background plate into the 7900HT instrument as follows:
a. From the plate document in the SDS software, click the Instrument tab.
b. From the lower portion tab, click the Real-Time tab.
c. From the Real-Time tab of the Instrument tabbed page, click Open/Close.
d. Place the background plate into the instrument tray as shown below.
PECY001DL3
Note TheA1positionislocatedinthetop-leftsideoftheinstrumenttray.
4 Click Start.
The 7900HT instrument begins the background run.
Remove the plate from the freezer and allow it to
thaw to room temperature.
a. Pipet deionized water to each well of the plate.
– If using a 384-well plate, add 20 µL perwell.
– If using a 96-well plate, add 50 µL per well.
b. Seal the plate using an optical adhesive cover
or optical flat caps.
Well A1
Position the plate so that the
bar code faces towards the
frontoftheinstrument
Note Before starting the run, the instrument may pause (up to 15 min) to heat the
heated cover to the appropriate temperature.
5 When the background run is complete and the Run Complete dialog box appears:
a. Click OK to close the dialog box.
b. Click Open/Close, and remove the background plate from the instrument tray.
c. Extract the background component as explained on page 7-16.
System Maintenance 7-15