ABI Prism® 7900HT Sequence Detection System ... - OpenWetWare

Preparing and Running a Single Plate
Pre-Run Checklist The following tasks must be complete to run a plate on the 7900HT instrument.
Plate Requirements See “Consumables and Disposables” on page D-3 for a complete list of ABI PRISM
consumables and ordering instructions.
Preparing the Plate
Done Check See Page
A background run has been performed in the last month 7-13
To prepare the plate:
A pure dye run has been performed in the 6 months 7-17
The instrument tray does not contain a plate
IMPORTANT The instrument tray must be empty to begin a run. If
the instrument tray contains a plate, eject and remove it before
continuing.
Step Action
1 Prepare the reactions in an ABI PRISM® Optical Reaction Plate by aliquoting
reagents, enzyme, and samples to the appropriate wells of an optical plate.
4-27
IMPORTANT The arrangement of the reactions (samples and assays) on the
plate must match the configuration of the corresponding plate document.
2 Seal the ABI PRISM Optical Reaction Plate with an ABI PRISM® Optical Adhesive
Cover or ABI PRISM® Optical Flat Cap Strips.
3 Briefly centrifuge the plate to collect the reactions at the bottom of the wells and to
eliminate any air bubbles that may be present.
4 Choose from the following:
Experiment Then…
Absolute Quantification/
Dissociation Curve Analysis
Run the plate as explained in “Running the
Plate” on page 4-24.
Allelic Discrimination a. Load the plate onto a designated thermal
cycler and perform the PCR.
b. Briefly centrifuge the plate to draw the
reactions to the bottom of the wells and to
eliminate any air bubbles that may have
formed during thermal cycling.
c. Run the plate as explained in “Running the
Plate” on page 4-24.
Run Setup and Basic Operation 4-23